RT Journal Article
SR Electronic
T1 A presynaptic spectrin network controls active zone assembly and neurotransmitter release
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 812032
DO 10.1101/812032
A1 Qi Wang
A1 Lindsey Friend
A1 Rosario Vicidomini
A1 Tae Hee Han
A1 Peter Nguyen
A1 Chun-Yuan Ting
A1 Mihaela Serpe
YR 2019
UL http://biorxiv.org/content/early/2019/10/21/812032.abstract
AB We have previously reported that Drosophila Tenectin (Tnc) recruits αPS2/βPS integrin to ensure structural and functional integrity at larval NMJs (Wang et al., 2018). In muscles, Tnc/integrin engages the spectrin network to regulate the size and architecture of synaptic boutons. In neurons, Tnc/integrin controls neurotransmitter release. Here we show that presynaptic Tnc/integrin modulates the synaptic accumulation of key active zone components, including the Ca2+ channel Cac and the active zone scaffold Brp. Presynaptic α-Spectrin appears to be both required and sufficient for the recruitment of Cac and Brp. We visualized the endogenous α-Spectrin and found that Tnc controls spectrin recruitment at synaptic terminals. Thus, Tnc/integrin anchors the presynaptic spectrin network and ensures the proper assembly and function of the active zones. Since pre- and postsynaptic Tnc/integrin limit each other, we hypothesize that this pathway links dynamic changes within the synaptic cleft to changes in synaptic structure and function.