RT Journal Article
SR Electronic
T1 High-depth resequencing reveals hybrid population and insecticide resistance characteristics of fall armyworm (<em>Spodoptera frugiperda</em>) invading China
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 813154
DO 10.1101/813154
A1 Lei Zhang
A1 Bo Liu
A1 Weigang Zheng
A1 Conghui Liu
A1 Dandan Zhang
A1 Shengyuan Zhao
A1 Pengjun Xu
A1 Kenneth Wilson
A1 Amy Withers
A1 Christopher M. Jones
A1 Judith A. Smith
A1 Gilson Chipabika
A1 Donald L. Kachigamba
A1 Kiwoong Nam
A1 Emmanuelle d’Alençon
A1 Bei Liu
A1 Xinyue Liang
A1 Minghui Jin
A1 Chao Wu
A1 Swapan Chakrabarty
A1 Xianming Yang
A1 Yuying Jiang
A1 Jie Liu
A1 Xiaolin Liu
A1 Weipeng Quan
A1 Guirong Wang
A1 Wei Fan
A1 Wanqiang Qian
A1 Kongming Wu
A1 Yutao Xiao
YR 2019
UL http://biorxiv.org/content/early/2019/10/21/813154.abstract
AB The rapid wide-scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report a 393.25-M chromosome-level genome assembly of fall armyworm with scaffold N50 of 13.3 M consisting of 23281 annotated protein-coding genes. Genome-wide resequencing of 105 samples from 16 provinces in China revealed that the fall armyworm population comprises a complex inter-strain hybrid, mainly with the corn-strain genetic background and less of the rice-strain genetic background, which highlights the inaccuracy of strain identification using mitochondrial or Tpi genes. An analysis of genes related to pesticide- and Bt-resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high, while remaining currently susceptible to Bt toxins. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, which is consistent with the results of molecular scanning of resistance-related genes, but are sensitive to genetically modified maize expressing Cry1Ab in field experiments. Additionally, we found that two mitochondrial fragments are inserted into the nuclear genome, and the insertion event occurred after the differentiation of the two strains. This study represents a valuable advancement toward the analysis of genetic differences among subpopulations and improving management strategies for fall armyworm.