RT Journal Article
SR Electronic
T1 Amino and PEG-Amino Graphene Oxide Grids Enrich and Protect Samples for High-resolution Single Particle Cryo-electron Microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 813972
DO 10.1101/813972
A1 Feng Wang
A1 Zanlin Yu
A1 Miguel Betegon
A1 Melody Campbell
A1 Tural Aksel
A1 Jianhua Zhao
A1 Sam Li
A1 Shawn M. Douglas
A1 Yifan Cheng
A1 David A. Agard
YR 2019
UL http://biorxiv.org/content/early/2019/10/22/813972.abstract
AB Cryo-EM samples prepared using the traditional methods often suffer from too few particles, poor particle distribution, or strongly biased orientation, or damage from the air-water interface. Here we report that functionalization of graphene oxide (GO) coated grids with amino groups concentrates samples on the grid with improved distribution and orientation. By introducing a PEG spacer, particles are kept away from both the GO surface and the air-water interface, protecting them from potential denaturation.