@article {Rashid786954, author = {Mubasher Rashid and Soumen Bera and Alexander B. Medvinsky and Gui-Quan Sun and Bai-Lian Li and Claudia Acquisti and Adnan Sljoka and Amit Chakraborty}, title = {Allosteric regulation of Glutamate dehydrogenase deamination activity}, elocation-id = {786954}, year = {2019}, doi = {10.1101/786954}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Glutamate dehydrogenase (GDH) is a key enzyme interlinking carbon and nitrogen metabolism. Recent discoveries of the GDH specific role in breast cancer, hyperinsulinism/hyperammonemia syndrome, and neurodegenerative diseases have reinvigorated interest on GDH regulation, which remains poorly understood despite extensive and long standing studies. Notwithstanding the growing evidence of the complexity of the allosteric network behind GDH regulation, a clear understanding of the enzyme dynamics involved in the process is limited to the activator Adenosine diphosphate (ADP) and the inhibitor Guanosine triphosphate (GTP). Presently, the identification of further factors involved in the allosteric network is paramount to deepen our understanding of the complex dynamics that regulate GDH enzymatic activity. Combining structural analyses of cryo-electron microscopy data with molecular dynamic simulations, here we show that the cofactor NADH is a key player in the GDH regulation process. Our structural analysis indicates that, binding to the regulatory sites in proximity of the antenna region, NADH acts as a positive allosteric modulator by enhancing both the affinity of GTP binding and inhibition of GDH catalytic activity. We further show that the binding of GTP to the NADH bound GDH produces a local conformational rearrangement that triggers an anticlockwise rotational motion of interlinked alpha-helices with specific tilted helical extension. This structural transition is a fundamental switch in the GDH enzymatic activity. It introduces a torsional stress, and the associated rotational shift in the Rossmann fold closes the catalytic cleft with consequent inhibition of the deamination process. These results shed new light on GDH regulation and may lay new foundation in the design of allosteric agents.}, URL = {https://www.biorxiv.org/content/early/2019/10/24/786954}, eprint = {https://www.biorxiv.org/content/early/2019/10/24/786954.full.pdf}, journal = {bioRxiv} }