RT Journal Article
SR Electronic
T1 ESCRT-mediated lysosome repair precedes lysophagy and promotes cell survival
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 313866
DO 10.1101/313866
A1 Maja Radulovic
A1 Antonino Bongiovanni
A1 Kay O. Schink
A1 Viola Nähse
A1 Eva M. Wenzel
A1 Frank Lafont
A1 Harald Stenmark
YR 2018
UL http://biorxiv.org/content/early/2018/05/03/313866.abstract
AB Although lysosomes perform a number of essential cellular functions, damaged lysosomes represent a potential hazard to the cell. Such lysosomes are therefore engulfed by autophagic membranes in the process known as lysophagy, which is initiated by recognition of luminal glycoprotein domains by cytosolic lectins such as Galectin-3. Here we show that, under various conditions that cause injury to the lysosome membrane, components of the endosomal sorting complex required for transport (ESCRT) machinery are recruited. This recruitment occurs before that of Galectin-3 and the lysophagy machinery. Subunits of the ESCRT-III complex show a particularly prominent recruitment, which depends on the ESCRT-I component TSG101 and the TSG101- and ESCRT-III-binding protein ALIX. Interference with ESCRT recruitment abolishes lysosome repair and causes otherwise reversible lysosome damage to become cell lethal. Vacuoles containing the intracellular pathogen Coxiella burnetii show reversible ESCRT recruitment, and interference with this recruitment reduces intravacuolar bacterial replication. We conclude that the cell is equipped with an endogenous mechanism for lysosome repair which protects against lysosomal damage-induced cell death but which also provides a potential advantage for intracellular pathogens.