RT Journal Article
SR Electronic
T1 A pan-plant protein complex map reveals deep conservation and novel assemblies
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 815837
DO 10.1101/815837
A1 McWhite, Claire D.
A1 Papoulas, Ophelia
A1 Drew, Kevin
A1 Cox, Rachael M.
A1 June, Viviana
A1 Dong, Oliver Xiaoou
A1 Kwon, Taejoon
A1 Wan, Cuihong
A1 Salmi, Mari L.
A1 Roux, Stanley J.
A1 Browning, Karen S.
A1 Chen, Z. Jeffrey
A1 Ronald, Pamela C.
A1 Marcotte, Edward M.
YR 2019
UL http://biorxiv.org/content/early/2019/10/24/815837.abstract
AB Plants are foundational to global ecological and economic systems, yet most plant proteins remain uncharacterized. Protein interaction networks often suggest protein functions and open new avenues to characterize genes and proteins. We therefore systematically determined protein complexes from 13 plant species of scientific and agricultural importance, greatly expanding the known repertoire of stable protein complexes in plants. Using co-fractionation mass spectrometry, we recovered known complexes, confirmed complexes predicted to occur in plants, and identified novel interactions conserved over 1.1 billion years of green plant evolution. Several novel complexes are involved in vernalization and pathogen defense, traits critical to agriculture. We also uncovered plant analogs of animal complexes with distinct molecular assemblies, including a megadalton-scale tRNA multi-synthetase complex. The resulting map offers the first cross-species view of conserved, stable protein assemblies shared across plant cells and provides a mechanistic, biochemical framework for interpreting plant genetics and mutant phenotypes.