PT  - JOURNAL ARTICLE
AU  - Jase Gehring
AU  - Jong Hwee Park
AU  - Sisi Chen
AU  - Matthew Thomson
AU  - Lior Pachter
TI  - Highly Multiplexed Single-Cell RNA-seq for Defining Cell Population and Transcriptional Spaces
AID  - 10.1101/315333
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 315333
4099  - http://biorxiv.org/content/early/2018/05/05/315333.short
4100  - http://biorxiv.org/content/early/2018/05/05/315333.full
AB  - We describe a universal sample multiplexing method for single-cell RNA-seq in which cells are chemically labeled with identifying DNA oligonucleotides. Analysis of a 96-plex perturbation experiment revealed changes in cell population structure and transcriptional states that cannot be discerned from bulk measurements, establishing a cost effective means to survey cell populations from large experiments and clinical samples with the depth and resolution of single-cell RNA-seq.