RT Journal Article
SR Electronic
T1 Highly Multiplexed Single-Cell RNA-seq for Defining Cell Population and Transcriptional Spaces
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 315333
DO 10.1101/315333
A1 Jase Gehring
A1 Jong Hwee Park
A1 Sisi Chen
A1 Matthew Thomson
A1 Lior Pachter
YR 2018
UL http://biorxiv.org/content/early/2018/05/05/315333.abstract
AB We describe a universal sample multiplexing method for single-cell RNA-seq in which cells are chemically labeled with identifying DNA oligonucleotides. Analysis of a 96-plex perturbation experiment revealed changes in cell population structure and transcriptional states that cannot be discerned from bulk measurements, establishing a cost effective means to survey cell populations from large experiments and clinical samples with the depth and resolution of single-cell RNA-seq.