TY - JOUR T1 - Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation JF - bioRxiv DO - 10.1101/591628 SP - 591628 AU - Fabiana Cerqueira Campos AU - Cynthia Dennis AU - Hervé Alégot AU - Cornelia Fritsch AU - Adam Isabella AU - Pierre Pouchin AU - Olivier Bardot AU - Sally Horne-Badovinac AU - Vincent Mirouse Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/10/28/591628.abstract N2 - How extracellular matrix participates to tissue morphogenesis is still an open question. In the Drosophila ovarian follicle, it has been proposed that after Fat2-dependent planar polarization of the follicle cell basal domain, oriented basement membrane (BM) fibrils and F-actin stress fibers constrain follicle growth, promoting its axial elongation. However, the relationship between BM fibrils and stress fibers and their respective impact on elongation are unclear. We found that Dystroglycan (Dg) and Dystrophin (Dys) are involved in BM fibril deposition. Moreover, they orient stress fibers, by acting locally and in parallel to Fat2. Nonetheless, Dg-Dys complex-mediated cell autonomous control of F-actin fibers orientation relies on the previous BM fibril deposition, indicating two distinct but interdependent functions. Thus, the Dg-Dys complex works as a critical organizer of the epithelial basal domain, regulating both F-actin and BM. Furthermore, BM fibrils act as a persistent cue for the orientation of stress fibers that are the main effector of elongation. ER -