RT Journal Article
SR Electronic
T1 Radial Glial from mammalian developing neocortex can perform symmetric proliferative divisions in vitro
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 707463
DO 10.1101/707463
A1 Mario Ledesma-Terrón
A1 Nuria Peralta-Cañadas
A1 David G. Míguez
YR 2019
UL http://biorxiv.org/content/early/2019/10/28/707463.abstract
AB Radial Glial progenitors in the mammalian developing neocortex have been shown to follow a deterministic differentiation program restricted to an asymmetric only mode of division. This feature contrasts with previous studies and with other developmental systems, such as the developing spinal cord, the retina, epidermis, airway epithelium, germline, and the intestine, where differentiation takes place based on probabilities that can change overtime and other modes of division are possible. Here, we combine experimental, computational and theoretical tools to show that Radial Glial cultured in vitro can divide symmetrically, and that the balance between the different modes of division, as well as the cell cycle length, can be modulated by external signals, such as Fibroblast Growth Factor. Our results suggest that the constraint of deterministic and asymmetric mode of division that Radial Glia exhibit in vivo may not be an inherent property of this particular cell type, but a feature induced by the complex organized pseudo-stratified structure of the mammalian developing neocortex.Significance Statement It is well established that, during the neurogenesis of the mammalian neocortex, Radial Glial can only divide asymmetrically. To understand the features that set this important restriction, we analyze in vitro cultures from mouse developing neocortex during the differentiation of Radial Glial into terminally differentiated neurons. Our results show that Radial Glial cultured in vitro also divide symmetrically. Moreover, the balance between the division modes can be modulated by external signals, such as Fibroblast Growth Factor. In conclusion, our results suggest that the constraint of deterministic and asymmetric mode of division of Radial Glial is not an inherent property of this particular cell type, but a feature induced by the complex organized pseudo-stratified structure of the mammalian developing neocortex.