TY - JOUR T1 - Exponential fluorescent amplification of individual RNAs using clampFISH probes JF - bioRxiv DO - 10.1101/222794 SP - 222794 AU - Sara H. Rouhanifard AU - Ian A. Mellis AU - Margaret Dunagin AU - Sareh Bayatpour AU - Orsolya Symmons AU - Allison Cote AU - Arjun Raj Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/05/07/222794.abstract N2 - Non-enzymatic, high-gain signal amplification methods with single-cell, single-molecule resolution are in great need. We present click-amplifying FISH (clampFISH) for the fluorescent detection of RNA that combines the specificity of oligonucleotides with bioorthogonal click chemistry in order to achieve high specificity and extremely high-gain (>400x) signal amplification. We show that clampFISH signal enables detection with low magnification microscopy and separation of cells by RNA levels via flow cytometry. Additionally, we show that the modular design of clampFISH probes enables multiplexing, that the locking mechanism prevents probe detachment in expansion microscopy, and that clampFISH works in tissue samples. ER -