RT Journal Article
SR Electronic
T1 Sparse recurrent excitatory connectivity in the microcircuit of the adult mouse and human cortex
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 292706
DO 10.1101/292706
A1 Stephanie C. Seeman
A1 Luke Campagnola
A1 Pasha A. Davoudian
A1 Alex Hoggarth
A1 Travis A. Hage
A1 Alice Bosma-Moody
A1 Christopher A. Baker
A1 Jung Hoon Lee
A1 Stefan Mihalas
A1 Corinne Teeter
A1 Andrew L. Ko
A1 Jeffrey G. Ojemann
A1 Ryder P. Gwinn
A1 Daniel L. Silbergeld
A1 Charles Cobbs
A1 John Phillips
A1 Ed Lein
A1 Gabe J. Murphy
A1 Christof Koch
A1 Hongkui Zeng
A1 Tim Jarsky
YR 2018
UL http://biorxiv.org/content/early/2018/05/08/292706.abstract
AB Generating a comprehensive description of cortical networks requires a large-scale, systematic approach. To that end, the Allen Institute is engaged in a pipeline project using multipatch electrophysiology, supplemented with 2-photon optogenetics, to characterize connectivity and synaptic signaling between classes of neurons in adult mouse and human cortex. We focus on producing results detailed enough for the generation of computational models and enabling comparison with future studies. Here we report our examination of intralaminar connectivity within each of several classes of excitatory neurons. We find that connections are sparse but present among all excitatory cell types and layers we sampled, with the most sparse connections in layers 5 and 6. Almost all mouse synapses exhibited short-term depression with similar dynamics. Synaptic signaling between a subset of layer 2/3 neurons; however, exhibited facilitation. These results contribute to a body of evidence describing recurrent excitatory connectivity as a conserved feature of cortical microcircuits.