RT Journal Article
SR Electronic
T1 Soluble cyanobacterial carotenoprotein as a robust antioxidant nanocarrier and delivery module
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 823880
DO 10.1101/823880
A1 Eugene G. Maksimov
A1 Alexey V. Zamaraev
A1 Evgenia Yu. Parshina
A1 Yury B. Slonimskiy
A1 Tatiana A. Slastnikova
A1 Alibek A. Abdrakhmanov
A1 Pavel A. Babaev
A1 Svetlana S. Efimova
A1 Olga S. Ostroumova
A1 Alexey V. Stepanov
A1 Anastasia V. Ryabova
A1 Thomas Friedrich
A1 Nikolai N. Sluchanko
YR 2019
UL http://biorxiv.org/content/early/2019/10/30/823880.abstract
AB To counteract oxidative stress, antioxidants including carotenoids are highly promising, yet their exploitation is drastically limited by the poor bioavailability and fast photodestruction, whereas current delivery systems are far from being efficient. Here we demonstrate that the recently discovered nanometer-sized water-soluble carotenoprotein from Anabaena (termed CTDH) transiently interacts with liposomes to efficiently extract carotenoids via carotenoid-mediated homodimerization, yielding violet-purple protein samples amenable to lyophilization and long-term storage. We characterize spectroscopic properties of the pigment-protein complexes and thermodynamics of liposome-protein carotenoid transfer and demonstrate the highly efficient delivery of echinenone form CTDH into liposomes. Most importantly, we show carotenoid delivery to membranes of mammalian cells, which provides protection from reactive oxygen species. The described carotenoprotein may be considered as part of modular systems for the targeted antioxidant delivery.Significance statement Carotenoids are excellent natural antioxidants but their delivery to vulnerable cells is challenging due to their hydrophobic nature and susceptibility to degradation. Thus, systems securing antioxidant stability and facilitating targeted delivery are of great interest for the design of medical agents. In this work, we have demonstrated that soluble cyanobacterial carotenoprotein can deliver echinenone into membranes of liposomes and mammalian cells with almost 70 % efficiency, which alleviates the induced oxidative stress. Our findings warrant the robustness of the protein-based carotenoid delivery for studies of carotenoid activities and effects on cell models.