PT - JOURNAL ARTICLE AU - Zhang, Liguo AU - Zhang, Yang AU - Chen, Yu AU - Gholamalamdari, Omid AU - Ma, Jian AU - Belmont, Andrew S. TI - TSA-Seq 2.0 reveals both conserved and variable chromosomal distances to nuclear speckles AID - 10.1101/824433 DP - 2019 Jan 01 TA - bioRxiv PG - 824433 4099 - http://biorxiv.org/content/early/2019/10/30/824433.short 4100 - http://biorxiv.org/content/early/2019/10/30/824433.full AB - TSA-Seq measures chromosomal distances from specific nuclear compartments genome-wide but requires ≥100 million cells. We report 10-20-fold increased sensitivity using TSA-Seq 2.0 which deliberately saturates protein-labeling but preserves distance mapping by the still unsaturated DNA-labeling. Mapping nuclear speckle distances in four cell lines reveals highly transcriptionally active, conserved speckle-associated chromosome domains but relative shifts of a small fraction of the genome that highly correlates with changes in gene expression.