TY - JOUR T1 - RNA Pol II Length and Disorder Enable Cooperative Scaling of Transcriptional Bursting JF - bioRxiv DO - 10.1101/825299 SP - 825299 AU - Porfirio Quintero-Cadena AU - Tineke L. Lenstra AU - Paul W. Sternberg Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/10/31/825299.abstract N2 - RNA Polymerase II contains a disordered C-terminal domain (CTD) whose length enigmatically correlates with genome size. The CTD is crucial to eukaryotic transcription, yet the functional and evolutionary relevance of this variation remains unclear. Here, we use smFISH, live imaging, and RNA-seq to investigate how CTD length and disorder influence transcription. We find that length modulates the size and frequency of transcriptional bursting. Disorder is highly conserved and mediates CTD-CTD interactions, an ability we show is separable from protein sequence and necessary for efficient transcription. We build a data-driven quantitative model, simulations of which recapitulate experiments and support CTD length promotes initial polymerase recruitment to the promoter but slows down its release from it, and that CTD-CTD interactions enable promoter recruitment of multiple polymerases. Our results reveal how these tunable parameters provide access to a range of transcriptional activity, offering a new perspective for the mechanistic significance of CTD length and disorder in transcription across eukaryotes. ER -