TY - JOUR T1 - Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow JF - bioRxiv DO - 10.1101/825224 SP - 825224 AU - René Riedel AU - Richard Addo AU - Marta Ferreira-Gomes AU - Gitta Anne Heinz AU - Frederik Heinrich AU - Jannis Kummer AU - Victor Greiff AU - Daniel Schulz AU - Cora Klaeden AU - Rebecca Cornelis AU - Ulrike Menzel AU - Stefan Kröger AU - Ulrik Stervbo AU - Ralf Köhler AU - Claudia Haftmann AU - Silvia Kühnel AU - Katrin Lehmann AU - Patrick Maschmeyer AU - Mairi McGrath AU - Sandra Naundorf AU - Stefanie Hahne AU - Özen Sercan-Alp AU - Francesco Siracusa AU - Jonathan Stefanowski AU - Melanie Weber AU - Kerstin Westendorf AU - Jakob Zimmermann AU - Anja E. Hauser AU - Sai T. Reddy AU - Pawel Durek AU - Hyun-Dong Chang AU - Mir-Farzin Mashreghi AU - Andreas Radbruch Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/10/31/825224.abstract N2 - Here we describe tissue-resident memory B lymphocytes of spleen and bone marrow. Single cell transcriptomes and B cell receptor repertoires identify several exclusive populations of isotype-switched memory B cells (Bsm) in murine spleen and bone marrow, and one interconnected population of 10-20%. A population of marginal zone-like Bsm is located exclusively in the spleen while a novel population of quiescent Bsm is located exclusively in the bone marrow. Cells of two further populations, present in both, spleen and bone marrow, differ in repertoire between the two organs, i.e. are resident as well. Finally, another interconnected population of Bsm of the B1 lineage is present in spleen and bone marrow. In the bone marrow, all Bsm individually dock onto VCAM1+ stromal cells, resting in terms of activation, proliferation and mobility. The discrete B cell memory of bone marrow may be key to rapid secondary humoral responses to systemic antigens.Bsmisotype-switched memory B cellBMbone marrowCGGchicken γ globulinIFAincomplete Freund’s adjuvanti.p.intraperitonealKLHkeyhole limpet hemocyaninLCMVlymphocytic choriomeningitis virusLPSlipopolysaccharideMANOVAMultivariate analysis of varianceNP4-hydroxy-3-nitrophenylacetyls.c.subcutaneousSPFspecific pathogen-freeTTtetanus toxoid ER -