PT - JOURNAL ARTICLE AU - Zezhou Zhao AU - Dillon C. Muth AU - Kathleen Mulka AU - Zhaohao Liao AU - Bonita H. Powell AU - Grace V. Hancock AU - Kelly A. Metcalf Pate AU - Kenneth W. Witwer TI - miRNA profiling of primate cervicovaginal lavage and extracellular vesicles reveals miR-186-5p as a potential retroviral restriction factor in macrophages AID - 10.1101/263947 DP - 2019 Jan 01 TA - bioRxiv PG - 263947 4099 - http://biorxiv.org/content/early/2019/10/31/263947.short 4100 - http://biorxiv.org/content/early/2019/10/31/263947.full AB - The goal of this study was to characterize extracellular vesicles (EVs) and miRNAs of primate cervicovaginal lavage (CVL) during the menstrual cycle and simian immunodeficiency virus (SIV) infection, and to determine if differentially regulated CVL miRNAs might influence retrovirus replication. CVL and peripheral blood were collected from SIV-infected and uninfected macaques. EVs were enriched by stepped ultracentrifugation and characterized thoroughly. miRNA profiles were assessed with a medium-throughput stem-loop/hydrolysis probe qPCR platform and validated by single qPCR assays. Hormone cycling was abnormal in infected subjects, but EV concentration correlated with progesterone concentration in uninfected subjects. miRNAs were present predominantly in the EV-depleted CVL supernatant. Only a small number of CVL miRNAs were found to vary during the menstrual cycle or SIV infection. Among them was miR-186-5p, which was depleted in retroviral infection. In experiments with infected macrophages in vitro, this miRNA inhibited HIV replication. These results provide further evidence for the potential of EVs and small RNAs as biomarkers or effectors of disease processes in the reproductive tract.