RT Journal Article
SR Electronic
T1 Identification of a novel tedizolid resistance mutation in <em>rpoB</em> of methicillin-resistant Staphylococcus aureus
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 827501
DO 10.1101/827501
A1 Tianwei Shen
A1 Kelsi Penewit
A1 Adam Waalkes
A1 Libin Xu
A1 Stephen J. Salipante
A1 Abhinav Nath
A1 Brian J. Werth
YR 2019
UL http://biorxiv.org/content/early/2019/11/01/827501.abstract
AB A tedizolid-resistant isolate of MRSA was selected by serial passage. Whole genome sequencing revealed only a single nucleotide variant in rpoB. Cross-resistance to linezolid, chloramphenicol, and quinupristin-dalfopristin was observed but susceptibility to other drugs including rifampin was unchanged. Models of the RNA-polymerase-ribosomal complex revealed that the mutated residue was unlikely to interact directly with the oxazolidinone binding site. This is the first time that rpoB mutation has been associated with resistance to the PhLOPSa antimicrobials.