PT  - JOURNAL ARTICLE
AU  - Caruso, Francesca Pia
AU  - Garofano, Luciano
AU  - D’Angelo, Fulvio
AU  - Yu, Kai
AU  - Tang, Fuchou
AU  - Yuan, Jinzhou
AU  - Zhang, Jing
AU  - Cerulo, Luigi
AU  - Bedognetti, Davide
AU  - Sims, Peter A.
AU  - Suvà, Mario
AU  - Su, Xiao-Dong
AU  - Lasorella, Anna
AU  - Iavarone, Antonio
AU  - Ceccarelli, Michele
TI  - A MAP of tumor-host interactions in glioma at single cell resolution
AID  - 10.1101/827758
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 827758
4099  - http://biorxiv.org/content/early/2019/11/01/827758.short
4100  - http://biorxiv.org/content/early/2019/11/01/827758.full
AB  - Single-cell RNA sequencing is the reference technique to characterize the heterogeneity of tumor microenvironment and can be efficiently used to discover cross-talk mechanisms between immune cells and cancer cells. We present a novel method, single cell Tumor-Host Interaction tool (scTHI), to identify significantly activated ligand-receptor interactions across clusters of cells from single-cell RNA sequencing data. We apply our approach to uncover the ligand-receptor interactions in glioma using six publicly available human glioma datasets encompassing 71 patients. We provide a comprehensive map of the signalling mechanisms between malignant cells and non-malignant cells in glioma uncovering potential novel therapeutic targets.