RT Journal Article
SR Electronic
T1 A MAP of tumor-host interactions in glioma at single cell resolution
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 827758
DO 10.1101/827758
A1 Caruso, Francesca Pia
A1 Garofano, Luciano
A1 D’Angelo, Fulvio
A1 Yu, Kai
A1 Tang, Fuchou
A1 Yuan, Jinzhou
A1 Zhang, Jing
A1 Cerulo, Luigi
A1 Bedognetti, Davide
A1 Sims, Peter A.
A1 Suvà, Mario
A1 Su, Xiao-Dong
A1 Lasorella, Anna
A1 Iavarone, Antonio
A1 Ceccarelli, Michele
YR 2019
UL http://biorxiv.org/content/early/2019/11/01/827758.abstract
AB Single-cell RNA sequencing is the reference technique to characterize the heterogeneity of tumor microenvironment and can be efficiently used to discover cross-talk mechanisms between immune cells and cancer cells. We present a novel method, single cell Tumor-Host Interaction tool (scTHI), to identify significantly activated ligand-receptor interactions across clusters of cells from single-cell RNA sequencing data. We apply our approach to uncover the ligand-receptor interactions in glioma using six publicly available human glioma datasets encompassing 71 patients. We provide a comprehensive map of the signalling mechanisms between malignant cells and non-malignant cells in glioma uncovering potential novel therapeutic targets.