RT Journal Article
SR Electronic
T1 Expansion Microscopy of Lipid Membranes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 829903
DO 10.1101/829903
A1 Karagiannis, Emmanouil D.
A1 Kang, Jeong Seuk
A1 Shin, Tay Won
A1 Emenari, Amauche
A1 Asano, Shoh
A1 Lin, Leanne
A1 Costa, Emma K.
A1 IMAXT Grand Challenge Consortium
A1 Marblestone, Adam H.
A1 Kasthuri, Narayanan
A1 Boyden, Edward S.
YR 2019
UL http://biorxiv.org/content/early/2019/11/04/829903.abstract
AB Lipids are fundamental building blocks of cells and their organelles, yet nanoscale resolution imaging of lipids has been largely limited to electron microscopy techniques. We introduce and validate a chemical tag that enables lipid membranes to be imaged optically at nanoscale resolution via a lipid-optimized form of expansion microscopy, which we call membrane expansion microscopy (mExM). mExM, via a novel post-expansion antibody labeling protocol, enables protein-lipid relationships to be imaged in organelles such as mitochondria, the endoplasmic reticulum, the nuclear membrane, and the Golgi apparatus. mExM may be of use in a variety of biological contexts, including the study of cell-cell interactions, intracellular transport, and neural connectomics.