RT Journal Article
SR Electronic
T1 High throughput, error corrected Nanopore single cell transcriptome sequencing
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 831495
DO 10.1101/831495
A1 Kevin Lebrigand
A1 Virginie Magnone
A1 Pascal Barbry
A1 Rainer Waldmann
YR 2019
UL http://biorxiv.org/content/early/2019/11/05/831495.abstract
AB Droplet-based high throughput single cell isolation techniques tremendously boosted the throughput of single cell transcriptome profiling experiments. However, those approaches only allow analysis of one extremity of the transcript after short read sequencing. We introduce an approach that combines Oxford Nanopore sequencing with unique molecular identifiers to obtain error corrected full length sequence information with the 10×Genomics single cell isolation system. This allows to examine differential RNA splicing and RNA editing at a single cell level.