RT Journal Article SR Electronic T1 Regenerative capacity in Drosophila imaginal discs is controlled by damage-responsive, maturity-silenced enhancers JF bioRxiv FD Cold Spring Harbor Laboratory SP 820605 DO 10.1101/820605 A1 Robin E. Harris A1 Michael J. Stinchfield A1 Spencer L. Nystrom A1 Daniel J. McKay A1 Iswar K. Hariharan YR 2019 UL http://biorxiv.org/content/early/2019/11/06/820605.abstract AB Like tissues of many organisms, Drosophila imaginal discs lose the ability to regenerate as they mature. This loss of regenerative capacity coincides with reduced damage-responsive expression of multiple genes needed for regeneration. Our previous work showed that two such genes, wg and Wnt6, are regulated by a single damage-responsive enhancer, which becomes progressively inactivated via Polycomb-mediated silencing as discs mature. Here we explore the generality of this mechanism, and identify numerous damage-responsive, maturity-silenced (DRMS) enhancers, some near genes known to be required for regeneration such as Mmp1, as well as near genes that we now show function in regeneration. Using a novel GAL4-independent tissue ablation system we characterize two DRMS-associated genes, apontic (apt), which curtails regeneration and CG9752/asperous (aspr), which promotes it. This mechanism of suppressing regeneration by silencing damage-responsive enhancers at multiple loci can be partially overcome by reducing activity of the chromatin regulator extra sex combs (esc).