TY - JOUR T1 - Transneuronal interactions facilitate axonal compartment formation JF - bioRxiv DO - 10.1101/834515 SP - 834515 AU - Bavat Bornstein AU - Idan Alyagor AU - Victoria Berkun AU - Hagar Meltzer AU - Fabienne Reh AU - Hadas Keren-Shaul AU - Eyal David AU - Thomas Riemensperger AU - Oren Schuldiner Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/11/07/834515.abstract N2 - Precise connectivity of neurites to subcellular domains is a conserved feature of complex circuits, but the mechanisms defining precise wiring are largely unknown. The Drosophila mushroom-body is uniquely poised to study subcellular wiring as the adult γ-lobe harbors five distinct compartments, defined by localized innervations of other neuronal populations. Here we identify mechanisms required for precise wiring of γ-axons: Dpr12 and its interacting protein DIP-δ are both enriched at the γ4/5 compartments. However, while Dpr12 is cell-autonomously required in γ-neurons, DIP-δ acts in dopaminergic neurons for the formation of the γ4/5 compartments and the correct network architecture. Furthermore, γ-axons grow into regions preoccupied by DIP-δ+ neurons suggesting that they innervate a prepatterned lobe. Thus, transneuronal interactions that mediate precise wiring are required for axon compartmentalization.One Sentence Summary Transneuronal interactions instruct precise wiring that enables localized innervation by specific neurons to distinct axonal compartments. ER -