RT Journal Article
SR Electronic
T1 Bacterial Swarming as a Protective Response to Intestinal Stress
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 759886
DO 10.1101/759886
A1 Weijie Chen
A1 Arpan De
A1 Hao Li
A1 Dana J. Lukin
A1 Wendy Szymczak
A1 Katherine Sun
A1 Libusha Kelly
A1 Justin R. Wright
A1 Regina Lamendella
A1 Subho Ghosh
A1 Daniel B. Kearns
A1 Zhen He
A1 Christian Jobin
A1 Xiaoping Luo
A1 Arjun Byju
A1 Shirshendu Chatterjee
A1 Beng San Yeoh
A1 Matam Vijay-Kumar
A1 Jay X. Tang
A1 Sridhar Mani
YR 2019
UL http://biorxiv.org/content/early/2019/11/07/759886.abstract
AB Bacterial swarming is a conserved and distinct form of bacterial motility that is often oppositely regulated and antagonistic to biofilm formation(1). To-date, while bacterial biofilms have been associated with pathogenesis and pathobiology of human diseases(2–4), there are very few examples of swarming behaviors that uniquely define or align with human pathophysiology(5–7). Here we report that swarming bacteria protects against intestinal inflammation in a murine model of colitis. Using feces in soft-agar plate assay we showed bacterial spreading harboring swarmers is highly predictive of the presence of intestinal stress in mice, pigs and humans. From murine feces, we isolated a novel Enterobacter swarming strain, SM3, which demonstrated significant protection from intestinal inflammation and promoted restitution in DSS-induced colitic mice. Known commensal swarmers also protected against intestinal inflammation when compared to swarming deficient isogenic mutants. Mechanistically, SM3 significantly reduced luminal oxygen concentration in colitic mice leading to a favorable anaerobic environment conducive to the growth of beneficial anaerobes. This work identifies a new paradigm in which intestinal stress, specifically inflammation, allows for emergence of swarming bacteria, which in turn has the ability to protect and heal from intestinal inflammation.One Sentence Summary Bacterial swarming reduce intestinal inflammation.