PT  - JOURNAL ARTICLE
AU  - Agathe Franck
AU  - Jeanne Lainé
AU  - Gilles Moulay
AU  - Michaël Trichet
AU  - Christel Gentil
AU  - Anaïs Fongy
AU  - Anne Bigot
AU  - Sofia Benkhelifa-Ziyyat
AU  - Emmanuelle Lacène
AU  - Mai Thao Bui
AU  - Guy Brochier
AU  - Pascale Guicheney
AU  - Vincent Mouly
AU  - Norma Romero
AU  - Catherine Coirault
AU  - Marc Bitoun
AU  - Stéphane Vassilopoulos
TI  - Clathrin plaques form mechanotransducing platforms
AID  - 10.1101/321885
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 321885
4099  - http://biorxiv.org/content/early/2018/05/14/321885.short
4100  - http://biorxiv.org/content/early/2018/05/14/321885.full
AB  - Large flat clathrin plaques are stable features of the plasma membrane associated with sites of strong adhesion suggesting that they could also play a role in force transduction. Here, we analyzed how clathrin plaques interact with the cytoskeleton and how they respond to mechanical cues in skeletal muscle myotubes. We show that branched actin networks surrounding clathrin plaques are directly regulated by dynamin 2, anchor intermediate filaments and sequester YAP/TAZ mechanotransducers at the plasma membrane. Dynamin 2, clathrin and desmin intermediate filaments are all required for basal YAP/TAZ nucleo/cytoplasmic distribution and efficient nuclear translocation in response to mechanical stimuli. Dynamin 2 mutations that are responsible for centronuclear myopathy in humans disorganize the desmin network and deregulate YAP/TAZ signaling both in vitro and in vivo. Thus, clathrin plaques and associated dynamin 2 act as sensors conveying mechanical cues and integrate cell signaling with cytoskeletal regulation.