RT Journal Article
SR Electronic
T1 Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 833319
DO 10.1101/833319
A1 Ruiz, Lidia
A1 Kaczmarska, Zuzanna
A1 Gomes, Tiago
A1 Aragón, Eric
A1 Torner, Carles
A1 Freier, Regina
A1 Bagiński, Błażej
A1 Martin-Malpartida, Pau
A1 de Martin Garrido, Natalia
A1 Márquez, José A.
A1 Cordeiro, Tiago
A1 Pluta, Radoslaw
A1 Macias, Maria J.
YR 2019
UL http://biorxiv.org/content/early/2019/11/07/833319.abstract
AB R-Smads are effectors of the transforming growth factor β (TGFβ) superfamily and along with Smad4 form trimers to interact with DNA. The 5GC-DNA complexes determined here by X-ray crystallography for Smad5 and Smad8 proteins corroborate that all MH1 domains bind SBE and 5GC sites similarly, although Smad2/3/4 MH1 domains bind DNA as monomers whereas Smad1/5/8 form helix-swapped dimers. To examine the relevance of the dimerization phenomenon and to exclude a possible crystallography-induced dimeric state, we studied these MH1 domains in solution. As in the crystals, Smad5/8 domains populate dimers and open monomers in equilibrium, whereas Smad/3/4 ones adopt monomeric closed conformations. We also found that swapping the loop1-sequence between Smad5 and Smad3 results in the chimera-DNA complex crystallizing as a monomer, revealing that the loop1-sequence determines the monomer/dimer propensity of Smad MH1-domains.We propose that distinct MH1-dimerization status of TGFβ and BMP activated Smads influences the interaction with specific loci genome-wide by distinct R-Smad and Smad4 complexes.Significance TGFβ- and BMP-activated R-Smads were believed to have different preferences with respect to the recognition of DNA motifs and to respond to specific activation inputs. However, recent results indicate that several types of R-Smads can be activated by similar receptors and that all Smads might recognize various DNA motifs. These results pose new questions as to why different types of R-Smads have been conserved for more than 500 million years if they could have a redundant function. They also raise questions as to how different Smad complexes recognize specific clusters of DNA motifs genome-wide.Here, using structural biology approaches, we elucidate some of the rules that help define dimers of Smad-DNA complexes and propose how these complexes could influence the recognition of specific cis regulatory elements genome-wide.Highlights R-Smads and Smad4 interact with GGCGCx and GTCT sites using a conserved binding mode.Functional differences of TGFβ- and BMP-activated R-Smads are not exclusively related to DNA specificity.Dimer/monomer propensities are detected in solution and in the absence of DNA.