RT Journal Article
SR Electronic
T1 Dysregulated RASGRP1 expression through RUNX1 mediated transcription promotes autoimmunity
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 656454
DO 10.1101/656454
A1 Matthijs Baars
A1 Thera Douma
A1 Dimitre R. Simeonov
A1 Darienne R. Myers
A1 Saikat Banerjee
A1 Kayla Kulhanek
A1 Susan Zwakenberg
A1 Marijke P. Baltissen
A1 Sytze de Roock
A1 Femke van Wijk
A1 Michiel Vermeulen
A1 Alexander Marson
A1 Jeroen P. Roose
A1 Yvonne Vercoulen
YR 2019
UL http://biorxiv.org/content/early/2019/11/08/656454.abstract
AB RasGRP1 is a Ras guanine nucleotide exchange factor, and an essential regulator of lymphocyte receptor signaling. In mice, Rasgrp1 deletion results in defective T lymphocyte development. RASGRP1-deficient patients suffer from immune deficiency, and the RASGRP1 gene has been linked to autoimmunity. However, how RasGRP1 levels are regulated, and if RasGRP1 dosage alterations contribute to autoimmunity remains unknown. We demonstrate that diminished Rasgrp1 expression caused defective T lymphocyte selection in C57BL/6 mice, and that the severity of inflammatory disease inversely correlates with Rasgrp1 expression levels. In patients with autoimmunity, active inflammation correlated with decreased RASGRP1 levels in CD4+ T cells. By analyzing H3K27 acetylation profiles in human T cells, we identified a RASGRP1 enhancer that harbors autoimmunity-associated SNPs. CRISPR-Cas9 disruption of this enhancer caused lower RasGRP1 expression, and decreased binding of RUNX1 and CBFB transcription factors. Analyzing patients with autoimmunity, we detected reduced RUNX1 expression in CD4+ T cells. Lastly, we mechanistically link RUNX1 to transcriptional regulation of RASGRP1 to reveal a key circuit regulating RasGRP1 expression, which is vital to prevent inflammatory disease.