PT  - JOURNAL ARTICLE
AU  - David Calderón Franco
AU  - Qingnan Lin
AU  - Mark C. M. van Loosdrecht
AU  - Ben Abbas
AU  - David G. Weissbrodt
TI  - Anticipating xenogenic pollution at the source: Impact of sterilizations on DNA release from microbial cultures
AID  - 10.1101/833228
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 833228
4099  - http://biorxiv.org/content/early/2019/11/08/833228.short
4100  - http://biorxiv.org/content/early/2019/11/08/833228.full
AB  - The dissemination of DNA and xenogenic elements across waterways is under scientific and public spotlight due to new gene-editing tools, such as do-it-yourself (DIY) CRISPR-Cas kits. Over decades, prevention of spread of genetically modified organisms (GMOs), antimicrobial resistances (AMR), and pathogens from transgenic systems has focused on microbial inactivation. However, sterilization methods have not been assessed for DNA release and integrity. Here, we investigated the fate of intracellular DNA from cultures of model prokaryotic (Escherichia coli) and eukaryotic (Saccharomyces cerevisiae) cells, commonly used as microbial hosts for genetic modifications, such as in white biotechnology. DNA release was tracked during exposure of these cultures to conventional sterilization methods. Autoclaving, disinfection with glutaraldehyde, and microwaving are used to inactivate broths, healthcare equipment, and GMOs produced at kitchen table. The results show that current sterilization methods are effective on microorganism inactivation but do not safeguard an aqueous residue exempt of biologically reusable xenogenic material, being regular autoclaving the most severe DNA-affecting method. Reappraisal of sterilization methods is required along with risk assessment on the emission of DNA fragments in urban systems and nature.