PT - JOURNAL ARTICLE AU - Guadalupe Martínez AU - Susana N. Diéguez AU - María B. Fernández Paggi AU - María B. Riccio AU - Denisa S. Pérez Gaudio AU - Julieta M. Decundo AU - Agustina Romanelli AU - Fabián A. Amanto AU - María O. Tapia AU - Alejandro L. Soraci TI - Effect of fosfomycin, <em>Cynara scolymus</em> extract, deoxynivalenol and their combinations on intestinal health of weaned piglets AID - 10.1101/323204 DP - 2018 Jan 01 TA - bioRxiv PG - 323204 4099 - http://biorxiv.org/content/early/2018/05/15/323204.short 4100 - http://biorxiv.org/content/early/2018/05/15/323204.full AB - Intestinal health of weaning piglets was studied after oral treatments with fosfomycin (FOS), Cynara scolymus extract (CSE), deoxynivalenol (DON) and their combinations. Piglets were divided in groups and received different treatments during 15 days, namely DON (1mg/kg of feed), FOS administered into the drinking water (30 mg/kg b.w.), CSE (300 g/ton of feed) and all possible combinations including a control group that received clean balanced diet. At day 15, three piglets from each group were euthanized and gastrointestinal tract samples were immediately taken to evaluate pH, bacteriology (enterobacteria and lactic acid bacteria), volatile fatty acids concentration (VFAs), disaccharidases activity (lactase, sucrase and maltase), histology (intestinal absorptive area [IAA] and goblet cells count) and adherence of bacteria to intestinal mucus. Animals receiving FOS and CSE treatments exhibited evident beneficial intestinal effects compared to animals receiving diets free from these compounds. This was revealed by a lower enterobacteria population together with a lower E/L, an enhanced production of butyric acid, an increased enzymatic activity (particularly maltase), and a greater IAA and goblet cells count along with an increase in pathogenic bacteria adherence to intestinal mucus. Interactions between both treatments resulted in similar beneficial effects as their individual administration. On the contrary, DON produced detrimental effects on intestinal health as a decrease was observed on volatile fatty acids production, enzymatic activity and goblet cells count in animals receiving diets containing sub- toxic concentrations of this mycotoxin. The knowledge of the intestinal effects of these compounds contributes to understand the physiological and pathological gut changes and their potential productive consequences.