RT Journal Article
SR Electronic
T1 Functional characterisation of gut microbiota and metabolism in Type 2 diabetes indicates that <em>Clostridiales</em> and <em>Enterococcus</em> could play a key role in the disease
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 836114
DO 10.1101/836114
A1 Marina Mora-Ortiz
A1 Alain Oregioni
A1 Sandrine P. Claus
YR 2019
UL http://biorxiv.org/content/early/2019/11/09/836114.abstract
AB There is growing evidence indicating that gut microbiota contributes to the development of metabolic syndrome and Type 2 Diabetes (T2D). The most widely-used model for T2D research is the leptin deficient db/db mouse model. Yet, a characterisation of the gut microbial composition in this model in relationship with the metabolism is lacking. The objectives of this study were to identify metabolomics and microbial modulations associated with T2D in the db/db mouse model. The majority of microbial changes observed included an increase of Enterobacteriaceae and a decrease of Clostridiales in diabetics. The metabolomics interrogation of caecum indicated a lower proteolytic activity in diabetics, who also showed higher Short-Chain Fatty Acid (SCFA) levels. In the case of faeces, the model identified 9 metabolites, the main ones were acetate, butyrate and Branched Chain Amino Acids (BCAAs). Finally, liver was the organ with more metabolic links with gut-microbiota followed by the Gut-Brain Axis (GBA). In conclusion, the interaction between Clostridiales and Enterococcus with caecal metabolism could play a key role in the onset and development of diabetes. Further studies should investigate whether the role of these bacteria is causal or co-occurring.BABile AcidsBCAABranched-Chain Amino Acidsdb/db micemice: BKS.Cg-Dock7&lt;m&gt; +/+ Lepr&lt;db&gt;/ J miceNMRNuclear Magnetic Resonance;TMAtrimethylamine;TMAOtrimethylamine-N-oxide;T2DType Two Diabetes;O-PLSOrthogonal Projections to Latent Structures;SCFAShort-Chain Fatty Acids.