RT Journal Article SR Electronic T1 Primary cilium-dependent cAMP/PKA signaling at the centrosome regulates neuronal migration JF bioRxiv FD Cold Spring Harbor Laboratory SP 765925 DO 10.1101/765925 A1 Stoufflet, Julie A1 Chaulet, Maxime A1 Doulazmi, Mohamed A1 Fouquet, Coralie A1 Dubacq, Caroline A1 Métin, Christine A1 Trembleau, Alain A1 Vincent, Pierre A1 Caillé, Isabelle YR 2019 UL http://biorxiv.org/content/early/2019/11/12/765925.abstract AB The primary cilium (PC) is a small centrosome-assembled organelle, protruding from the surface of most eukaryotic cells. It plays a key role in cell migration, but the underlying mechanisms are unknown. Here, we show that the PC regulates neuronal migration via cAMP production activating centrosomal Protein Kinase A (PKA). Biosensor live-imaging revealed a periodic cAMP hotspot at the centrosome of embryonic, postnatal and adult migrating neurons. Genetic ablation of the PC, or knock-down of ciliary Adenylate Cyclase 3, caused hotspot disappearance and migratory defects, with defective centrosome/nucleus coupling and altered nucleokinesis. Delocalization of PKA from the centrosome phenocopied the migratory defects. Our results show that the PC and centrosome form a single cAMP-signaling unit dynamically regulating migration, further highlighting the centrosome as a signaling hub.The primary cilium regulates neuronal migration via cyclic AMP production activating Protein Kinase A at the centrosome