TY - JOUR T1 - Serial protein crystallography in an electron microscope JF - bioRxiv DO - 10.1101/682575 SP - 682575 AU - Robert Bücker AU - Pascal Hogan-Lamarre AU - Pedram Mehrabi AU - Eike C. Schulz AU - Lindsey A. Bultema AU - Yaroslav Gevorkov AU - Wolfgang Brehm AU - Oleksandr Yefanov AU - Dominik Oberthür AU - Günther H. Kassier AU - R. J. Dwayne Miller Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/11/13/682575.abstract N2 - We describe a new method for serial electron diffraction of protein nanocrystals using a conventional scanning/transmission electron microscope (S/TEM). Randomly dispersed crystals are mapped, and dose-efficient diffraction patterns measured at each identified crystal position for structure determination. Each crystal is measured in a single orientation without rotation. The automated workflow is suitable for high-throughput applications with acquisition rates of up to 1 kHz at a high hit fraction. A dose fractionation scheme allows for minimization of radiation damage effects and inherently optimal acquisition settings. We demonstrate this method by solving the structure of crystalline granulovirus occlusion bodies and lysozyme to a resolution of 1.55 Å and 1.80 Å, respectively. Our method promises to provide rapid high-quality structure determination for many classes of materials with minimal sample consumption, using readily available instrumentation. ER -