RT Journal Article SR Electronic T1 Repression of divergent noncoding transcription by a sequence-specific transcription factor JF bioRxiv FD Cold Spring Harbor Laboratory SP 314310 DO 10.1101/314310 A1 Wu, Andrew CK A1 Patel, Harshil A1 Chia, Minghao A1 Moretto, Fabien A1 Frith, David A1 Snijders, Ambrosius P A1 van Werven, Folkert J YR 2018 UL http://biorxiv.org/content/early/2018/05/17/314310.abstract AB Many active eukaryotic gene promoters exhibit divergent noncoding transcription, but the mechanisms restricting expression of these transcripts are not well understood. Here we demonstrate how a sequence-specific transcription factor represses divergent noncoding transcription at highly expressed genes in yeast. We find that depletion of the transcription factor Rap1 induces noncoding transcription in a large fraction of Rap1 regulated gene promoters. Specifically, Rap1 prevents transcription initiation at cryptic promoters near its binding sites, which is uncoupled from transcription regulation in the protein-coding direction. We further provide evidence that Rap1 acts independently of chromatin-based mechanisms to repress cryptic or divergent transcription. Finally, we show that divergent transcription in the absence of Rap1 is elicited by the RSC chromatin remodeller. We propose that a sequence-specific transcription factor limits access of basal transcription machinery to regulatory elements and adjacent sequences that act as divergent cryptic promoters, thereby providing directionality towards productive transcription.