PT  - JOURNAL ARTICLE
AU  - Enriqueta Garcia-Gutierrez
AU  - Paula M. O’Connor
AU  - Ian J. Colquhoun
AU  - Natalia M. Vior
AU  - Juan Miguel Rodríguez
AU  - Melinda J. Mayer
AU  - Paul D. Cotter
AU  - Arjan Narbad
TI  - Production of multiple bacteriocins, including the novel bacteriocin gassericin M, by <em>Lactobacillus gasseri</em> LM19, a strain isolated from human milk
AID  - 10.1101/841254
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 841254
4099  - http://biorxiv.org/content/early/2019/11/13/841254.short
4100  - http://biorxiv.org/content/early/2019/11/13/841254.full
AB  - Bacteriocins are antimicrobial peptides produced by bacteria and their production by health-promoting microbes is regarded as a desirable probiotic trait. We found that Lactobacillus gasseri LM19, a strain isolated from human milk, exhibits antagonistic activity against different enteropathogens and produces several bacteriocins, including a novel bacteriocin, gassericin M. These bacteriocins were purified from culture and synthesised to investigate their activity and potential synergy. L. gasseri LM19 was tested in a complex environment mimicking human colon conditions where it not only survived but expressed the seven bacteriocin genes and produced short chain fatty acids. Metagenomic analysis of these in vitro colon cultures showed that co-inoculation of L. gasseri LM19 with Clostridium perfringens gave profiles with more similarity to controls than to vessels inoculated with C. perfringens alone. This makes L. gasseri LM19 an interesting candidate for further study for maintaining homeostasis in the gut environment.