PT  - JOURNAL ARTICLE
AU  - Kinshuk Mitra
AU  - William N. Feist
AU  - Simone Anfossi
AU  - Enrique Fuentes-Mattei
AU  - Maria Ines Almeida
AU  - Jean J. Kim
AU  - George A. Calin
AU  - Aryeh Warmflash
TI  - S-MiRAGE: A quantitative, secreted RNA-based reporter of gene expression and cell persistence
AID  - 10.1101/325092
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 325092
4099  - http://biorxiv.org/content/early/2018/05/17/325092.short
4100  - http://biorxiv.org/content/early/2018/05/17/325092.full
AB  - Non-destructive measurements of cell persistence and gene expression are crucial for longitudinal research studies and for prognostic assessment of cell therapies. Here we describe S-MiRAGE, a platform that utilizes small secreted RNA molecules as sensitive and quantitatively accurate reporters of cellular processes. We demonstrate the utility of S-MiRAGE by monitoring the differentiation status of human embryonic stem cells in vitro, and tumor growth in a mouse model in vivo.