RT Journal Article
SR Electronic
T1 Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports its Classification as a Novel Species
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 844340
DO 10.1101/844340
A1 Chappell, Joseph G.
A1 Tsoleridis, Theocharis
A1 Onianwa, Okechukwu
A1 Drake, Gabby
A1 Ashpole, Ian
A1 Dobbs, Phillipa
A1 Edema, William
A1 Kumi-Ansah, Frederick
A1 Bennett, Malcolm
A1 Tarlinton, Rachael E
A1 Ball, Jonathan K
A1 McClure, C. Patrick
YR 2019
UL http://biorxiv.org/content/early/2019/11/16/844340.abstract
AB Hantaviruses are a diverse group of single-stranded, negative-sensed RNA viruses, known to cause sporadic outbreaks of potentially fatal human disease. To date, only two Orthohantavirus species have been detected in the UK - Seoul virus and Tatenale. Whilst Seoul is known to be pathogenic in humans, only partial fragments of Tatenale have been recovered, precluding any accurate analysis of its phylogeny or potential pathogenicity. To overcome this shortfall we used a degenerate primer PCR method to identify Tatenale-infection in rodents living in two separate locations in the UK. PCR positive samples were then subjected to either unbiased high-throughput sequencing or overlapping PCR product sequencing to recover the complete coding sequence of the Tatenale virus. This analysis provided in-depth insight into the evolutionary origins of this recently identified UK Orthohantavirus and unequivocally showed that Tatenale virus meets the established criteria for classification as a novel species. Crucially, our data will facilitate in vitro investigation into the zoonotic potential of Tatenale virus.