RT Journal Article
SR Electronic
T1 Transcriptional reprogramming of distinct peripheral sensory neuron subtypes after axonal injury
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 838854
DO 10.1101/838854
A1 William Renthal
A1 Ivan Tochitsky
A1 Lite Yang
A1 Yung-Chih Cheng
A1 Emmy Li
A1 Riki Kawaguchi
A1 Daniel H. Geschwind
A1 Clifford J. Woolf
YR 2019
UL http://biorxiv.org/content/early/2019/11/16/838854.abstract
AB Primary somatosensory neurons are specialized to transmit specific types of sensory information through differences in cell size, myelination, and the expression of distinct receptors and ion channels, which together define their transcriptional and functional identity. By transcriptionally profiling sensory ganglia at single-cell resolution, we find that different somatosensory neuronal subtypes undergo a remarkably consistent and dramatic transcriptional response to peripheral nerve injury that both promotes axonal regeneration and suppresses cell identity. Successful axonal regeneration leads to a restoration of neuronal cell identity and the deactivation of the growth program. This injury-induced transcriptional reprogramming requires Atf3, a transcription factor which is induced rapidly after injury and is necessary for axonal regeneration and functional recovery. While Atf3 and other injury-induced transcription factors are known for their role in reprogramming cell fate, their function in mature neurons is likely to facilitate major adaptive changes in cell function in response to damaging environmental stimuli.