RT Journal Article SR Electronic T1 Fatherhood alters gene expression within the MPOA JF bioRxiv FD Cold Spring Harbor Laboratory SP 258111 DO 10.1101/258111 A1 Adele M. H. Seelke A1 Jessica M. Bond A1 Trent C. Simmons A1 Nikhil Joshi A1 Matthew L. Settles A1 Danielle Stolzenberg A1 Mijke Rhemtulla A1 Karen L. Bales YR 2018 UL http://biorxiv.org/content/early/2018/05/19/258111.abstract AB Female parenting is obligate in mammals, but fathering behavior among mammals is rare. Only 3–5% of mammalian species exhibit biparental care, including humans, and mechanisms of fathering behavior remain sparsely studied. However, in species where it does exist, paternal care is often crucial to the survivorship of offspring. The present study is the first to identify new gene targets linked to the experience of fathering behavior in a biparental species using RNA sequencing. In order to determine the pattern of gene expression within the medial preoptic area that is specifically associated with fathering behavior, we identified differentially expressed genes in male prairie voles (Microtus ochrogaster) that experienced one of three social conditions: virgin males, pair bonded males, and males with fathering experience. Differentially expressed genes from each comparison (i.e., Virgin vs Paired, Virgin vs Fathers, and Paired vs Fathers) were evaluated using the Gene Ontology enrichment analysis, and Kegg pathways analysis to reveal metabolic pathways associated with specific differentially expressed genes. Using these tools, we identified a group of genes that are differentially expressed in voles with different amounts of social experience. These genes are involved in a variety of processes, with particular enrichment in genes associated with immune function, metabolism, synaptic plasticity, and the remodeling of dendritic spines. The identification of these genes and processes will lead to novel insights into the biological basis of fathering behavior.