PT  - JOURNAL ARTICLE
AU  - Rebekka Sontowski
AU  - Michael Gerth
AU  - Sandy Richter
AU  - Axel Gruppe
AU  - Martin Schlegel
AU  - Nicole van Dam
AU  - Christoph Bleidorn
TI  - Infections patterns and fitness effects of <em>Rickettsia</em> and <em>Sodalis</em> symbionts in the green lacewing <em>Chrysoperla carnea</em>
AID  - 10.1101/327130
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 327130
4099  - http://biorxiv.org/content/early/2018/05/21/327130.short
4100  - http://biorxiv.org/content/early/2018/05/21/327130.full
AB  - Endosymbionts are wide-spread among insects and can play an essential role in host ecology. The common green lacewing (Chrysoperla carnea s. str.) is a neuropteran insect species which is widely used as a biological pest control. We screened for endosymbionts in natural and laboratory populations of the green lacewing using diagnostic PCR amplicons. We found the endosymbiont Rickettsia to be very common in all screened populations, whereas a so far uncharacterized Sodalis strain was solely found in laboratory populations. The new Sodalis strain was characterized using a whole genome shotgun approach. Its draft genome revealed an approximate genome size of 4.3 Mbp and the presence of 5213 coding sequences. Phylogenomic analyses indicated that this bacterium is the sister taxon of S. praecaptivus. In an experimental approach, we found a negative impact of Sodalis on the reproduction success of the green lacewing. Co-infections with Rickettsia and Sodalis caused an even higher decrease of reproductive success than single Sodalis infections. In contrast, no significant fitness differences were found in Rickettsia infected green lacewings compared to uninfected lacewings. The Rickettsia/Sodalis/Ch. carnea system presents a promising model to study evolutionary endosymbiont-host interactions in Neuroptera and endosymbiont-endosymbiont interactions in general. The economic and ecological importance of green lacewings in biological pest control warrants a more profound understanding of its biology, which might be strongly influenced by symbionts.