Regular ArticleCytokinin Oxidase/Cytokinin Dehydrogenase Assay: Optimized Procedures and Applications
References (31)
- et al.
Isolation of a gene encoding a glycosylated cytokinin oxidase from maize
Biochem. Biophys. Res. Commun.
(1999) - et al.
A colorimetric assay for cytokinin oxidase
Anal. Biochem.
(1995) - et al.
Cytokinin oxidases in mosses. 1. Metabolism of kinetin and benzyladenine in vivo
J. Plant Physiol.
(1990) - et al.
Cytokinin oxidases in mosses. 1. Metabolism of kinetin and benzyladenine in vitro
J. Plant Physiol.
(1990) - et al.
The effect of metyrapone on cytokinin ([8-14C]benzylaminopurine) metabolism in mature green tomato pericarp
Biochem. Biophys. Res. Commun.
(1987) - et al.
Cyclic assay for nicotinamide adenine dinucleotides: NaCl precipitation and ethanol solubilization of the reduced tetrazolium
Anal. Biochem.
(1997) - et al.
Enzymatic cycling assay for d-carnitine determination
Anal. Biochem.
(1999) - et al.
NADP(+)-isocitrate dehydrogenase gene expression and isozyme activity during citrus fruit development
Plant Sci.
(2000) - et al.
Specific detection of quinoproteins by redox-cycling staining
J. Biol. Chem.
(1991) - et al.
Cytokinin oxidase: Biochemical features and physiological significance
Physiol. Plant.
(1994)
Cytokinin oxidase or dehydrogenase? Mechanism of the cytokinin degradation in plants
Eur. J. Biochem.
Molecular and biochemical characterization of a cytokinin oxidase from maize
Plant Physiol.
Cytokinin metabolism and action
Annu. Rev. Plant Physiol. Plant Mol. Biol.
Degradation of cytokinins by cytokinin oxidases in plants
Plant Growth Reg.
Cytokinin oxidase from Zea mays: Purification, cDNA cloning and expression in moss protoplasts
Plant J.
Cited by (76)
OsCKX2 regulates phosphate deficiency tolerance by modulating cytokinin in rice
2022, Plant ScienceCitation Excerpt :The hydroponic nutrient solution was changed every 2 days throughout the treatment. The measurement of CKX activity was performed according to the methods described previously [25,26]. Seedling samples of OE, KO, and WT plants under NP and LP conditions were homogenized and prepared as a slurry by mixing chilled Tris/HCl buffer (0.2 M, pH 8.0), containing 1 mM phenylmethylsulphonyl fluoride (PMSF) and 0.3% Triton X-100.
Blue light suppression alters cytokinin homeostasis in wheat leaves senescing under shading stress
2018, Plant Physiology and BiochemistryCitation Excerpt :Cell debris was removed by centrifugation at maximum speed for 20 min at 4 °C. The supernatant was used for the enzymatic activity determination by the end-point assay with 4-aminophenol (Frébort et al., 2002). The reaction mixture of 0.6 mL final volume contained 100 μL of the protein extract, 200 μM of iP as substrate and 0.5 mM of 2,6-dichlorophenolindophenol (DCPIP) as electron donor, in 200 mM McIlvaine buffer (citric acid/disodium hydrogen phosphate, pH = 6.8).
Maize cytokinin dehydrogenase isozymes are localized predominantly to the vacuoles
2016, Plant Physiology and BiochemistryCitation Excerpt :Protein concentration in clarified extract and concentrated media was quantified using the Bradford method (Bradford, 1976). CKX activity in cell lysates and in culture media was measured by the end point method (Frébort et al., 2002). Aliquots of cell lysate (1–50 μL) or concentrated media (2–100 μL) were incubated at 37 °C for 2–16 h in a reaction mixture (total volume of 0.6 mL) consisting of 250 mM McIlvaine buffer (pH 7.5), 0.125 mM dichlorophenolindophenol, and 250 μM substrate iP.
Transgenic barley overexpressing a cytokinin dehydrogenase gene shows greater tolerance to drought stress
2016, New BiotechnologyCitation Excerpt :The blast results were then filtered to accept only those results having >80% identity, and 20,108 genes of the reference genome were definitely assigned to the Zea mays genes. The CKX activity was determined spectrophotometrically [30] using 0.5 mM 2,3-dimetoxy-5-methyl-1,4-benzoquinone as an electron acceptor and 0.25 mM substrate iPR in McIlvaine buffer (pH 5.5). All measurements were performed in three biological replicates.
Cytokinin Oxidase/Dehydrogenase as an Important Target for Increasing Plant Productivity
2024, Cytology and Genetics
- 1
To whom correspondence should be addressed. Fax: ++420-68-5221332. E-mail: [email protected].