The Role of Aging, High Fat Diet and Blue Light Exposure in an Experimental Mouse Model for Basal Laminar Deposit Formation

doi:10.1006/exer.2002.2047

Experimental Eye Research

Volume 75, Issue 5, November 2002, Pages 543-553

https://doi.org/10.1006/exer.2002.2047 Get rights and content

Abstract

We sought to investigate the role of aging as a susceptibility factor for the capacity of dietary fat intake to increase the development of subretinal deposits. Mice of various ages (2, 9 and 16 months) were fed a normal diet or a diet high in saturated and unsaturated fats for a total four and a half months. Some eyes were also exposed to non-phototoxic levels of blue-green light. The outer retina and choroid were examined by light and transmission electron microscopy, and the characteristics, frequency and severity of subRPE deposits was determined. Aged mice fed normal diets developed only very mild subretinal deposits. However, many eyes of mice aged 9 months or older at the time of initiation of diet developed frequent basal laminar deposits of moderate severity, and only 16 month old mice developed more severe deposits after exposure to blue-green light. Some eyes in this older group also developed endothelial invasion into Bruch's membrane. None of the eyes developed classic drusen or linear deposits. These observations demonstrate that age increases the capacity of dietary fat, especially in the presence of environmental light, to induce subRPE deposits.

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^f1: Address correspondence to: Scott W. Cousins, Department of Ophthalmology, University of Miami School of Medicine, PO Box 016880, Miami, FL 33138, U.S.A. E-mail: [email protected]

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Regular ArticleThe Role of Aging, High Fat Diet and Blue Light Exposure in an Experimental Mouse Model for Basal Laminar Deposit Formation

Abstract

Mech. Ageing Dev

J. Photochem. Photobiol. B

Ophthalmology

Ophthalmology

J. Invest. Dermatol

J. Biol. Chem

Am. J. Clin. Nutr

Ophthalmology

Methods Enzymol

Light and aging as co-factors in retinal pigment epithelial disease

Clinico-morphologic correlations of drusen of Bruch's membrane

Trans. Am. Ophthalmol. Soc

Interaction of dietary protein, cholesterol and age on lipid metabolism of the rat

Br. J. Nutr

Essential fatty acids: the importance of n-3 fatty acids in the retina and brain

Nutr. Rev

Membrane blebbing in RPE cells: differentiating between injury stimuli and regulatory cofactors [ARVO Abstract]

Invest. Ophthalmol. Vis. Sci

Photoreceptor loss in age-related macular degeneration

Invest. Ophthalmol. Vis. Sci

Basal linear deposits and large drusen are specific for early age-related maculopathy

Arch. Ophthalmol

Spare the rods, save the cones in aging and age-related maculopathy

Invest. Ophthalmol. Vis. Sci

Lipid peroxidation in rod outer segments. Role of hydroxyl radical and lipid hydroperoxides

Invest. Ophthalmol. Vis. Sci

Region and age-dependent variation in susceptibility of the human retina to lipid peroxidation

Invest. Ophthalmol. Vis. Sci

Effect of diet on the fatty acid and molecular species composition of dog retina phospholipids

Lipids

Ultrastructural changes in Bruch's membrane of apolipoprotein E-deficient mice

Invest. Ophthalmol. Vis. Sci

Cell loss in the aging retina. Relationship to lipofuscin accumulation and macular degeneration

Invest. Ophthalmol. Vis. Sci

Lipofuscin and other lysosomal storage deposits in the retinal pigment epithelium

Age-related changes in plasma and tissue fatty acid composition in Fischer 344 rats

Biochem. Molec. Biol. Int

Hypercholesterolemia does not alter retinal sterol composition or compromise retinal structure or function in Apolipoprotein E (Apo E) - Knockout mice [ARVO Abstract]

Invest. Ophthalmol. Vis. Sci

Vitamin E in macular and peripheral tissues of the human eye

Curr. Eye Res

Degenerative and related disorders of the retina and choroid

Histopathology of age-related macular degeneration

Molec. Vis

Regular Article
The Role of Aging, High Fat Diet and Blue Light Exposure in an Experimental Mouse Model for Basal Laminar Deposit Formation