Journal of Molecular Biology
CommunicationStructural Mimicry and Enhanced Immunogenicity of Peptide Epitopes Displayed on Filamentous Bacteriophage: The V3 Loop of HIV-1 gp120
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Anti-V2 antibody deficiency in individuals infected with HIV-1 in Cameroon
2019, VirologyCitation Excerpt :We analyzed 3–9 gp120 sequences per subject. Sequence analysis and alignment was performed using DNASTAR and Clustal W (di Marzo Veronese et al., 1994). The gp120 nucleotide sequences of 12 plasma viruses from HIV-1 infected individuals with deficient and cross-reactive anti-V2 Abs are available from GenBank with the accession numbers: MH632751– MH632762.
Phagemid vectors for phage display: Properties, characteristics and construction
2012, Journal of Molecular BiologyCitation Excerpt :Only when there is no gene III in the genome of helper phage would five copies be present in the progeny phages.89 However, the progenies of type VIII phagemid can append much more than five copies of foreign proteins,90 even hundreds or thousands of copies, which are determined by the genome size of phagemids when the gene VIII of helper phage is missing. With the increase in the copies of displayed foreign proteins in this displaying system, the length of displayed peptides would decrease drastically to a few or a dozen amino acids.91
Serum anti-p53 antibody detection in carcinomas and the predictive values of serum p53 antibodies, carcino-embryonic antigen and carbohydrate antigen 12-5 in the neoadjuvant chemotherapy treatment for III stage non-small cell lung cancer patients
2011, Clinica Chimica ActaCitation Excerpt :Phage display technology was once used for tumor-associated antigens and autoantibodies' screening during biopanning [18]. Portefaix et al. and di Marzo Veronese et al. [19,20] compared the immunogenicity of phage-displayed peptides and the synthetic peptides in ELISA; the outcome indicated that phage-displayed peptides had advantage over synthetic peptides inasmuch as they effectively mimicked the natural epitope structure and were correctly recognized by antibodies. With the purpose of increasing detection sensitivity and making a full use of hybrid phages' advantage, we designed the p53-ELISA and phage-ELISA double detection system to measure the titer of s-p53 Abs in cancers.
Fc receptor-mediated, antibody-dependent enhancement of bacteriophage lambda-mediated gene transfer in mammalian cells
2008, VirologyCitation Excerpt :Since gene expression is enhanced by 10- to 50-fold by disruption of the microtubule network, it appears likely that most of the internalized phage-immune complexes are ultimately destined for degradation, under normal circumstances. The ability of phage-immune complexes to efficiently transduce FcγR-bearing mammalian cells has important implications for phage-mediated gene transfer (Eguchi et al., 2001; Hajitou et al., 2006; Ivanenkov et al., 1999; Kassner et al., 1999; Larocca et al., 1999; Sathaliyawala et al., 2006; Zanghi et al., 2007), and for applications in which phage vectors are being used to elicit immune responses to encoded or displayed antigens (Chen et al., 2001; Clark and March, 2004; di Marzo Veronese et al., 1994; Irving et al., 2001; Jepson and March, 2004; Minenkova et al., 1993; Sathaliyawala et al., 2006). For example, in vaccine applications, it may be beneficial to generate phage-immune complexes in vitro with the goal of targeting vector to FcγR-expressing antigen presenting cells (APC) in vivo.
The detection of serum anti-p53 antibodies from patients with gastric carcinoma in China
2007, Cancer Detection and Prevention