Regular ArticleControl of Expression, Glycosylation, and Secretion of HIV-1 gp120 by Homologous and Heterologous Signal Sequences
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2021, Protein Expression and PurificationCitation Excerpt :In addition, the SP may associate with calnexin to deter glycoprotein folding [11]. These findings support the replacement of the native gpl20 signal sequence with a heterologous one to promote gp120 secretion [8–10,43]. Despite of using none-native SP, recombinant gp120 expression remained ~1 mg/L in mammalian cells [10,43,44].
HIV-1 Envelope Glycan Composition as a Key Determinant of Efficient Virus Transmission via DC-SIGN and Resistance to Inhibitory Lectins
2019, iScienceCitation Excerpt :Biosynthesis of HIV-1 Env is initiated by translation of the signal peptide (SP), which transports nascent protein to the ER and Golgi pathways. Notably, HIV-1 Env SP is highly variable and contains several basic residues in its N-terminal region (Figure 4A); these features are unique to Env SPs and are not seen among SPs of other viral or cellular glycoproteins (Li et al., 1994). We previously showed that SP sequences influence HIV-1 Env glycosylation; particularly, mutations at SP positions 8, 12, and 15 were each found to change the oligosaccharide contents of Env glycans, which, in turn, affected virus neutralization by antibody (Upadhyay et al., 2018).
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