Abstract
This chapter presents a review of fluorescence correlation spectroscopy (FCS), an experimental technique with single-molecule sensitivity, which is based on the analysis of fluctuations of fluorescence intensity detected from a tiny volume. Correlation functions of fluorescence fluctuations can provide information on the translational and rotational diffusion of fluorophores, dynamics of singlet–triplet transitions, chemical reactions, flow, and active transport of fluorescent molecules. A detailed theoretical description of the fluorescence correlation and cross-correlation technique is followed by a discussion of various experimental aspects of FCS, including the choice of instrumentation and fluorophores, sample- and setup-related nonidealities and possible artifacts, statistical accuracy, and approaches to the analysis of FCS data. Additionally, some FCS application aspects are addressed, including the quantitative determination of translational diffusion coefficients and the use of FCS in studies of (bio)polymers and phospholipid membranes. The chapter concludes with an overview of both well-established and currently emerging varieties of FCS and related methods, including the use of two-photon excitation and the application of total internal reflection, nanoapertures, and stimulated emission depletion to confine the detection volume; the use of higher-order correlations; application of time-resolved and time-gated detection; multifocal and CCD-based FCS; and image correlation and scanning FCS techniques.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Magde D, Elson E, Webb WW (1972) Phys Rev Lett 29:705
Elson EL, Magde D (1974) Biopolymers 13:1
Magde D, Elson EL, Webb WW (1974) Biopolymers 13:29
Koppel DE (1974) Phys Rev A 10:1938
Ehrenberg M, Rigler R (1974) Chem Phys 4:390
Aragón SR, Pecora R (1975) Biopolymers 14:119
Aragón SR, Pecora R (1976) J Chem Phys 64:1791
Koppel DE, Axelrod D, Schlessinger J, Elson EL, Webb WW (1976) Biophys J 16:1315
Magde D, Webb WW, Elson EL (1978) Biopolymers 17:361
Berne BJ, Pecora R (1976) Dynamic light scattering. Wiley, New York
Schaefer DW, Berne BJ (1972) Phys Rev Lett 28:475
Schaefer DW (1973) Science 180:1293
Elson EL, Webb WW (1975) Annu Rev Biophys Bioeng 4:311
Magde D (1976) Q Rev Biophys 9:35
Ehrenberg M, Rigler R (1976) Q Rev Biophys 9:69
Elson EL (1985) Annu Rev Phys Chem 36:379
Thompson N (1991) In: Lakowicz JR (ed) Topics in fluorescence spectroscopy, vol 1: techniques. Plenum Press, New York, p 337
Lakowicz JR (1999) Principles of fluorescence spectroscopy, 2nd edn. Kluwer Academic/Plenum Press, New York
O'Connor DV, Phillips D (1984) Time-correlated single photon counting. Academic, London
Rigler R, Mets Ü, Widengren J, Kask P (1993) Eur Biophys J 22:169
Eigen M, Rigler R (1994) Proc Nat Acad Sci USA 91:5740
Rigler R, Elson E (eds) (2001) Fluorescence correlation spectroscopy: theory and applications. Springer, Berlin
Schwille P (2001) Cell Biochem Biophys 34:383
Hess ST, Huang S, Heikal AA, Webb WW (2002) Biochemistry 41:697
Widengren J, Mets Ü (2002) In: Zander C, Enderlein J, Keller RA (eds) Single molecule detection in solution. Wiley-VCH, Berlin, p 69
Thompson NL, Lieto AM, Allen NW (2002) Curr Opin Struct Biol 12:634
Krichevsky O, Bonnet G (2002) Rep Prog Phys 65:251
Bacia K, Schwille P (2003) Methods 29:74
Haustein E, Schwille P (2003) Methods 29:153
Gösch M, Rigler R (2005) Adv Drug Deliv Rev 57:169
Vukojević V, Pramanik A, Yakovleva T, Rigler R, Terenius L, Bakalkin G (2005) Cell Mol Life Sci 62:535
Kahya N, Schwille P (2006) Mol Membr Biol 23:29
Bacia K, Kim SA, Schwille P (2006) Nat Methods 3:83
Loudon R (2000) The quantum theory of light, 3rd edn. Oxford University Press, Oxford
Saleh B (1978) Photoelectron statistics. Springer, Berlin
Landau LD, Lifshitz EM (1980) Statistical physics, part 1, 3rd edn. Butterworth-Heinemann, Oxford
Mazo RM (2002) Brownian motion: fluctuations, dynamics and applications. Oxford University Press, Oxford
Kinjo M, Rigler R (1995) Nucleic Acids Res 23:1795
Schwille P, Oehlenschläger F, Walter NG (1996) Biochemistry 35:10182
Meseth U, Wohland T, Rigler R, Vogel H (1999) Biophys J 76:1619
Gardiner CW (2004) Handbook of stochastic methods for physics, chemistry and the natural sciences, 3rd edn, Chap 8. Springer, Berlin
Tsien RY (1998) Annu Rev Biochem 67:509
Haupts U, Maiti S, Schwille P, Webb WW (1998) Proc Nat Acad Sci USA 95:13573
Widengren J, Terry B, Rigler R (1999) Chem Phys 249:259
Bonnet G, Krichevsky O, Libchaber A (1998) Proc Nat Acad Sci USA 95:8602
Chattopadhyay K, Saffarian S, Elson EL, Frieden C (2002) Proc Nat Acad Sci USA 99:14171
Neuweiler H, Doose S, Sauer M (2005) Proc Nat Acad Sci USA 102:16650
Kim J, Doose S, Neuweiler H, Sauer M (2006) Nucl Acid Res 34:2516
Kawski A (1993) CR Anal Chem 23:459
Yardley JT, Specht LT (1976) Chem Phys Lett 37:543
Hoshikawa H, Asai H (1985) Biophys Chem 22:167
Scalettar BA, Klein MP, Hearst JE (1987) Biopolymers 26:1287
Kask P, Piksarv P, Mets Ü, Pooga M, Lippmaa E (1987) Eur Biophys J 14:257
Widengren J, Mets Ü, Rigler R (1999) Chem Phys 250:171
Tsay JM, Doose S, Weiss S (2006) J Am Chem Soc 128:1639
Hinze G, Diezemann G, Basché T (2004) Phys Rev Lett 93:203001
Wei CYJ, Kim YH, Darst RK, Rossky PJ, Vanden Bout DA (2005) Phys Rev Lett 95:173001
Rosenberg SA, Quinlan ME, Forkey JN, Goldman YE (2005) Acc Chem Res 38:583
Vanden Bout DA, Deschenes LA (2006) Science 312:195
Uji-i H, Melnikov SM, Deres A, Bergamini G, De Schryver F, Herrmann A, Müllen K, Enderlein J, Hofkens J (2006) Polymer 47:2511
Barcellona ML, Gammon S, Hazlett T, Digman MA, Gratton E (2004) Microsc Res Tech 65:205
Zondervan R, Kulzer F, van der Meer H, Disselhorst JAJM, Orrit M (2006) Biophys J 90:2958
Carmichael HJ, Walls DF (1976) J Phys B 9:L43
Kimble HJ, Dagenais M, Mandel L (1977) Phys Rev Lett 39:691
Kask P, Piksarv P, Mets Ü (1985) Eur Biophys J 12:163
Basché T, Moerner WE, Orrit M, Talon H (1992) Phys Rev Lett 69:1516
Bernard J, Fleury L, Talon H, Orrit M (1993) J Chem Phys 98:850
Widengren J, Mets Ü, Rigler R (1995) J Phys Chem 99:13368
Kitson SC, Jonsson P, Rarity JG, Tapster PR (1998) Phys Rev A 58:620
Mets Ü (2001) In: Rigler R, Elson E (eds) Fluorescence correlation spectroscopy: theory and applications. Springer, Berlin, p 346
Felekyan S, Kühnemuth R, Kudryavtsev V, Sandhagen C, Becker W, Seidel CAM (2005) Rev Sci Instrum 76:083104
Mets Ü, Widengren J, Rigler R (1997) Chem Phys 218:191
Griffin WG, Pusey PN (1979) Phys Rev Lett 43:1100
Kam Z, Rigler R (1982) Biophys J 39:7
Rička J, Binkert T (1989) Phys Rev A 39:2646
Schwille P, Meyer-Almes F-J, Rigler R (1997) Biophys J 72:1878
Heinze KG, Koltermann A, Schwille P (2000) Proc Nat Acad Sci USA 97:10377
Weidemann T, Wachsmuth M, Tewes M, Rippe K, Langowski J (2002) Single Mol 3:49
Bacia K (2005) PhD Thesis, Technische Universität Dresden
Kim S, Heinze KG, Bacia K, Waxham MN, Schwille P (2005) Biophys J 88:4319
Hwang LC, Gösch M, Lasser T, Wohland T (2006) Biophys J 91:715
Thews E, Gerken M, Eckert R, Zäpfel J, Tietz C, Wrachtrup J (2005) Biophys J 89:2069
Müller BK, Zaychikov E, Bräuchle C, Lamb DC (2005) Biophys J 89:3508
Burkhardt M, Heinze KG, Schwille P (2005) Opt Lett 30:2266
Hwang LC, Leutenegger M, Gösch M, Lasser T, Rigler P, Meier W, Wohland T (2006) Opt Lett 31:1310
Palmer AG III, Thompson NL (1987) Biophys J 51:339
Asai H (1980) Japan J Appl Phys 19:2279
Lenne P-F, Colombo D, Giovannini H, Rigneault H (2002) Single Mol 4:194
Brinkmeier M (1996) PhD Thesis, Technische Universität Braunschweig
Brinkmeier M, Dörre K, Stephan J, Eigen M (1999) Anal Chem 71:609
Brinkmeier M (2001) In: Rigler R, Elson E (eds) Fluorescence correlation spectroscopy: theory and applications. Springer, Berlin, p 379
Dittrich PS, Schwille P (2002) Anal Chem 74:4472
Gösch M, Blom H, Holm J, Heino T, Rigler R (2000) Anal Chem 72:3260
Lumma D, Best A, Gansen A, Feuillebois F, Rädler JO, Vinogradova OI (2003) Phys Rev E 67:056313
Kuricheti KK, Buschmann V, Weston KD (2004) Appl Spectrosc 58:1180
Dittrich PS, Müller B, Schwille P (2004) Phys Chem Chem Phys 6:4416
Dittrich PS, Schäfer SP, Schwille P (2005) Biophys J 89:3446
Brister PC, Kuricheti KK, Buschmann V, Weston KD (2005) Lab Chip 5:785
Köhler RH, Schwille P, Webb WW, Hanson MR (2000) J Cell Sci 113:3921
Bouchaud J-P, Georges A (1990) Phys Rep 195:127
Schwille P, Korlach J, Webb WW (1999) Cytometry 36:176
Schwille P, Haupts U, Maiti S, Webb WW (1999) Biophys J 77:2251
Wachsmuth M, Waldeck W, Langowski J (2000) J Mol Biol 298:677
Weiss M, Hashimoto H, Nilsson T (2003) Biophys J 84:4043
Weiss M, Elsner M, Kartberg F, Nilsson T (2004) Biophys J 87:3518
Fatin-Rouge N, Starchev K, Buffle J (2004) Biophys J 86:2710
Banks DS, Fradin C (2005) Biophys J 89:2960
Szymański J, Patkowski A, Gapiński J, Wilk A, Hołyst R (2006) J Phys Chem B 110:7367
Masuda A, Ushida K, Riken TO (2005) Biophys J 88:3584
Wawrezinieck L, Rigneault H, Marguet D, Lenne P-F (2005) Biophys J 89:4029
Mandelbrot BB, van Ness JW (1968) SIAM Rev 10:422
Metzler R, Klafter J (2000) Phys Rep 339:1
Wang KG, Lung CW (1990) Phys Lett A 151:119
Feder TJ, Brust-Mascher I, Slattery JP, Baird B, Webb WW (1996) Biophys J 70:2767
Lutz E (2001) Phys Rev E 64:051106
Lim SC, Muniandy SV (2002) Phys Rev E 66:021114
Hilfer R (ed) (1999) Applications of fractional calculus in physics. World Scientific, Singapore
Saichev AI, Zaslavsky GM (1997) Chaos 7:753
Barkai E (2001) Phys Rev E 63:046118
Feller W (1970) An introduction to probability theory and its applications, vol 2. Wiley, New York
Hac AE, Seeger HM, Fidorra M, Heimburg T (2005) Biophys J 88:317
Schütz GJ, Schindler H, Schmidt T (1997) Biophys J 73:1073
Schneider J, Rička J, Binkert T (1988) Rev Sci Instrum 59:588
Eid JS, Müller JD, Gratton E (2000) Rev Sci Instrum 71:361
Benda A, Hof M, Wahl M, Patting M, Erdmann R, Kapusta P (2005) Rev Sci Instrum 76:033106
Becker W, Bergmann A, Haustein E, Petrasek Z, Schwille P, Biskup C, Kelbauskas L, Benndorf K, Klöcker N, Anhut T, Riemann I, König K (2006) Microsc Res Tech 69:186
Weisshart K, Jüngel V, Briddon SJ (2004) Curr Pharm Biotechnol 5:135
Woggon U (1996) Optical properties of semiconductor quantum dots. Springer, Berlin
Gaponenko SV (1998) Optical properties of semiconductor nanocrystals. Cambridge University Press, Cambridge
Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, Sundaresan G, Wu AM, Gambhir SS, Weiss S (2005) Science 307:538
Larson DR, Zipfel WR, Williams RM, Clark SW, Bruchez MP, Wise FW, Webb WW (2003) Science 300:1434
Kapitonov AM, Stupak AP, Gaponenko SV, Petrov EP, Rogach AL, Eychmüller A (1999) J Phys Chem B 103:10109
Zenkevich E, Cichos F, Shulga A, Petrov EP, Blaudeck T, von Borczyskowski C (2005) J Phys Chem B 109:8679
Petrov EP, Cichos F, von Borczyskowski C (2006) J Luminesc 119–120:412
Doose S, Tsay JM, Pinaud F, Weiss S (2005) Anal Chem 77:2235
Yao J, Larson DR, Vishwasrao HD, Zipfel WR, Webb WW (2005) Proc Nat Acad Sci USA 102:14284
Jin T, Fujii F, Sakata H, Tamura M, Kinjo M (2005) Chem Commun 2829
Zhang J, Campbell RE, Ting AY, Tsien RY (2002) Nat Rev Mol Cell Biol 3:906
Baird GS, Zacharias DA, Tsien RY (2000) Proc Nat Acad Sci USA 97:11984
Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA, Tsien RY (2002) Proc Nat Acad Sci USA 99:7877
Shaner NC, Campbell RE, Steinbach PA, Giepmans BNG, Palmer AE, Tsien RY (2004) Nat Biotechnol 22:1567
Ando R, Hama H, Yamamoto-Hino M, Mizuno H, Miyawaki A (2002) Proc Nat Acad Sci USA 99:12651
Ando R, Mizuno H, Miyawaki A (2004) Science 306:1370
Schwille P, Kummer S, Heikal AA, Moerner WE, Webb WW (2000) Proc Nat Acad Sci USA 97:151
Schenk A, Ivanchenko S, Röcker C, Wiedenmann J, Nienhaus GU (2004) Biophys J 86:384
Hess ST, Heikal AA, Webb WW (2004) J Phys Chem B 108:10138
Cotlet M, Goodwin PM, Waldo GS, Werner JH (2006) ChemPhysChem 7:250
Schäfer SP, Dittrich PS, Petrov EP, Schwille P (2006) Microsc Res Tech 69:210
Dedecker P, Hotta J, Ando R, Miyawaki A, Engelborghs Y, Hofkens J (2006) Biophys J 91:L45
Phillies GDJ (1975) Biopolymers 14:499
Axelrod D, Koppel DE, Schlessinger J, Elson E, Webb WW (1976) Biophys J 16:1055
Saxton MJ, Jacobson K (1997) Annu Rev Biophys Biomol Struct 26:373
Abney JR, Scalettar BA, Hackenbrock CR (1990) Biophys J 58:261
Abney JR, Scalettar BA (1995) Biophys Chem 57:27
Doi M, Edwards SF (1986) The theory of polymer dynamics. Oxford University Press, Oxford
Teraoka I (2002) Polymer solutions: an introduction to physical properties. Wiley, New York
Scalettar BA, Hearst JE, Klein MP (1989) Macromolecules 22:4550
Wilk A, Gapinski J, Patkowski A, Pecora R (2004) J Chem Phys 121:10794
Liu R, Gao X, Adams J, Oppermann W (2005) Macromolecules 38:8845
Nishimura G, Kinjo M (2004) Anal Chem 76:1963
Gregor I, Patra D, Enderlein J (2005) ChemPhysChem 6:164
Nagy A, Wu J, Berland KM (2005) J Biomed Opt 10:044015
Nagy A, Wu J, Berland KM (2005) Biophys J 89:2077
Enderlein J (1996) Phys Lett A 221:427
Widengren J, Rigler R (1996) Bioimaging 4:149
Eggeling C, Widengren J, Rigler R, Seidel CAM (1998) Anal Chem 70:2651
Dittrich PS, Schwille P (2001) Appl Phys B 73:829
Niesner R, Roth W, Gericke K-H (2004) ChemPhysChem 5:678
Eggeling C, Widengren J, Brand L, Schaffer J, Felekyan S, Seidel CAM (2006) J Phys Chem A 110:2979
Delon A, Usson Y, Derouard J, Biben T, Souchier C (2006) Biophys J 90:2548
Widengren J, Thyberg P (2005) Cytometry A 68:101
Ashkin A (1997) Proc Nat Acad Sci USA 94:4853
Osborne MA, Balasubramanian S, Furey WS, Klenerman D (1998) J Phys Chem B 102:3160
Ding Y, Meng FB, Chen B, Ma H, Lei J, Chen DY (2001) Acta Phys Sin 50:2269
Chirico G, Fumagalli C, Baldini G (2002) J Phys Chem B 106:2508
Meng F, Ma H (2005) J Phys Chem B 109:5580
Hosokawa C, Yoshikawa H, Masuhara H (2005) Phys Rev E 72:021408
Hosokawa C, Yoshikawa H, Masuhara H (2006) Jpn J Appl Phys 45:L453
Haustein E (2006) PhD Thesis, Technische Universität Dresden
Palmer AG III, Thompson NL (1989) Appl Opt 28:1214
Qian H, Elson EL (1991) Appl Opt 30:1185
Shaw PJ, Rawlins DJ (1991) J Microsc 163:151
Juškaitis R, Wilson T (1998) J Microsc 189:8
Visser TD, Wiersma SH (1994) J Opt Soc Am A 11:599
Török P, Wilson T (1997) Opt Commun 137:127
Dorn R, Quabis S, Leuchs G (2003) J Mod Opt 50:1917
Haeberlé O (2004) Opt Commun 235:1
Dimova R, Aranda S, Bezlyepkina N, Nikolov V, Riske KA, Lipowsky R (2006) J Phys Condens Matter 18:S1151
Hess ST, Webb WW (2002) Biophys J 83:2300
Enderlein J, Gregor I, Patra D, Dertinger T, Kaupp UB (2005) ChemPhysChem 6:2324
Benda A, Beneš M, Mareček V, Lhotský A, Hermens WT, Hof M (2003) Langmuir 19:4120
Enderlein J, Gregor I, Patra D, Fitter J (2004) Curr Pharm Biotechnol 5:155
Chattopadhyay K, Saffarian S, Elson EL, Frieden C (2005) Biophys J 88:1413
Zettl H, Häfner W, Böker A, Schmalz H, Lanzendörfer M, Müller AHE, Krausch G (2004) Macromolecules 37:1917
Dravins D, Faria D, Nilsson B (2000) Proc SPIE 4008:298
Burstyn HC, Sengers JV (1983) Phys Rev A 27:1071
Burstyn HC (1980) Rev Sci Instrum 51:1431
Campbell L (1992) Rev Sci Instrum 63:5794
Zhao M, Jin L, Chen B, Ding Y, Ma H, Chen D (2003) Appl Opt 42:4031
Enderlein J, Gregor I (2005) Rev Sci Instrum 76:033102
Schätzel K (1986) Appl Phys B 41:95
Palmer AG III, Thompson NL (1989) Rev Sci Instrum 60:624
Apanasovich VV, Paltsev SV (1995) J Opt Soc Am B 12:1550
Nishimura G, Kinjo M (2005) Appl Opt 44:3458
Schätzel K, Drewel M, Stimac S (1988) J Mod Opt 35:711
Bevington P, Robinson DK (2003) Data reduction and error analysis for the physical sciences, 3rd edn. McGraw-Hill, New York
Qian H (1990) Biophys Chem 38:49
Kask P, Günther R, Axhausen P (1997) Eur Biophys J 25:163
Wohland T, Rigler R, Vogel H (2001) Biophys J 80:2087
Saffarian S, Elson EL (2003) Biophys J 84:2030
Schätzel K (1990) Quantum Opt 2:287
Harrison D, Fisch MR (1996) Langmuir 12:6691
Marriott FHC, Pope JA (1954) Biometrika 41:390
Jakeman E, Pike ER, Swain S (1971) J Phys A 4:517
Ries J, Schwille P (2006) Biophys J 91:1915
Petrov EP, Ohrt T, Winkler RG, Schwille P (2006) Phys Rev Lett 97:258101
Phillies GDJ (1996) Rev Sci Instrum 67:3423
Kojro Z, Riede A, Schubert M, Grill W (1999) Rev Sci Instrum 70:4487
Enderlein J, Gregor I, Patra D, Fitter J (2005) J Fluoresc 15:415
Starchev K, Zhang J, Buffle J (1998) J Colloid Interface Sci 203:189
Gennerich A, Schild D (2000) Biophys J 79:3294
Gennerich A, Schild D (2002) Biophys J 83:510
Widengren J, Schwille P (2000) J Phys Chem A 104:6416
Skakun VV, Hink MA, Digris AV, Engel R, Novikov EG, Apanasovich VV, Visser AJWG (2005) Eur Biophys J 34:323
Tikhonov AN, Arsenin VY (1977) Solutions of ill-posed problems. Winston, Washington
Hansen PC (1998) Rank-deficient and discrete ill-posed problems: numerical aspects of linear inversion. SIAM, Philadelphia
Provencher SW (1982) Comput Phys Commun 27:213
Livesey AK, Brochon JC (1987) Biophys J 52:693
Siemiarczuk A, Wagner B, Ware WR (1990) J Phys Chem 94:1661
Langowski J, Bryan R (1991) Macromolecules 24:6346
Gakamsky DM, Goldin AS, Petrov EP, Rubinov AN (1992) Biophys Chem 44:47
Petrov EP, Kruchenok JV, Rubinov AN (1999) J Fluoresc 9:111
Björling S, Kinjo M, Földes-Papp Z, Hagman E, Thyberg P, Rigler R (1998) Biochemistry 37:12971
Starchev K, Buffle J, Pérez E (1999) J Colloid Interface Sci 213:479
Rigler R, Pramanik A, Jonasson P, Kratz G, Jansson OT, Nygren P-Å, Ståhl S, Ekberg K, Johansson B-L, Uhlén S, Uhlén M, Jörnvall H, Wahren J (1999) Proc Nat Acad Sci USA 96:13318
Pramanik A, Olsson M, Langel Ü, Bartfai T, Rigler R (2001) Biochemistry 40:10839
Módos K, Galántai R, Bárdos-Nagy I, Wachsmuth M, Tóth K, Fidy J, Langowski J (2004) Eur Biophys J 33:59
Sengupta P, Garai K, Balaji J, Periasamy N, Maiti S (2003) Biophys J 84:1977
Davies B (2002) Integral transforms and their applications, 3rd edn. Springer, New York
Jankevics H, Prummer M, Izewska P, Pick H, Leufgen K, Vogel H (2005) Biochemistry 44:11676
Krouglova T, Vercammen J, Engelborghs Y (2004) Biophys J 87:2635
Culbertson CT, Jacobson SC, Ramsey JM (2002) Talanta 56:365
Moore AW Jr, Jorgenson JW (1993) Anal Chem 65:3550
Paul PH, Garguilo MG, Rakestraw DJ (1998) Anal Chem 70:2459
Jaffiol R, Blancquaert Y, Delon A, Derouard J (2006) Appl Opt 45:1225
Dertinger T, Pacheco V, von der Hocht I, Hartmann R, Gregor I, Enderlein J (2007) Chem Phys Chem 8:433
Blancquaert Y, Delon A, Derouard J, Jaffiol R (2006) Proc SPIE 6191:61910B
Hattori M, Shimizu H, Yokoyama H (1996) Rev Sci Instrum 67:4064
Hansen RL, Zhu XR, Harris JM (1998) Anal Chem 70:1281
Asai H, Ando T (1976) J Phys Soc Japan 50:1527
Rigneault H, Lenne P-F (2003) J Opt Soc Am B 20:2203
Knowles MK, Grassman TJ, Marcus AH (2000) Phys Rev Lett 85:2837
Brochard F, Lennon JF (1975) J Phys France 36:1035
Granek R (1997) J Phys II France 7:1761
Lumma D, Keller S, Vilgis T, Rädler JO (2003) Phys Rev Lett 90:218301
Shusterman R, Alon S, Gavrinyov T, Krichevsky O (2004) Phys Rev Lett 92:048303
Winkler RG, Keller S, Rädler JO (2006) Phys Rev E 73:041919
Harnau L, Winkler RG, Reineker P (1996) J Chem Phys 104:6355
Eimer W, Pecora R (1991) J Chem Phys 94:2324
Sorlie SS, Pecora R (1990) Macromolecules 23:487
Smith DE, Perkins TT, Chu S (1996) Macromolecules 29:1372
Nkodo AE, Garnier JM, Tinland B, Ren H, Desruisseaux C, McCormick LC, Drouin G, Slater GW (2001) Electrophoresis 22:2424
Petrov EP, Schwille P (2005) Biophys J 88:524A
Fradin C, Abu-Arish A, Granek R, Elbaum M (2003) Biophys J 84:2005
Berland KM, So PTC, Gratton E (1995) Biophys J 68:694
Diaspro A, Chirico G, Collini M (2005) Q Rev Biophys 38:97
Sánchez SA, Gratton E (2005) Acc Chem Res 38:469
Thompson NL, Burghardt TP, Axelrod D (1981) Biophys J 33:435
Starr TE, Thompson NL (2001) Biophys J 80:1575
Lieto AM, Cush RC, Thompson NL (2003) Biophys J 85:3294
Lieto AM, Thompson NL (2004) Biophys J 87:1268
Pero JK, Haas EM, Thompson NL (2006) J Phys Chem B 110:10910
Axelrod D, Hellen EH, Fulbright RM (1992) In: Lakowicz JR (ed) Topics in fluorescence spectroscopy, vol 3. Plenum Press, New York, p 289
Ruckstuhl T, Seeger S (2004) Opt Lett 29:569
Hassler K, Leutenegger M, Rigler P, Rao R, Rigler R, Gösch M, Lasser T (2005) Opt Express 13:7415
Leutenegger M, Blom H, Widengren J, Eggeling C, Gösch M, Leitgeb RA, Lasser T (2006) J Biomed Opt 11:040502
Levene MJ, Korlach J, Turner SW, Foquet M, Craighead HG, Webb WW (2003) Science 299:682
Wenger J, Rigneault H, Dintinger H, Marguet D, Lenne P-F (2006) J Biol Phys 32:SN1
Samiee KT, Moran-Mirabal JM, Cheung YK, Craighead HG (2006) Biophys J 90:3288
Rigneault H, Capoulade J, Dintinger J, Wenger J, Bonod N, Popov E, Ebbesen TW, Lenne P-F (2005) Phys Rev Lett 95:117401
Kastrup L, Blom H, Eggeling C, Hell SW (2005) Phys Rev Lett 94:178104
Palmer AG III, Thompson NL (1987) Biophys J 52:257
Thompson NL, Mitchell JL (2001) In: Rigler R, Elson E (eds) Fluorescence correlation spectroscopy: theory and applications. Springer, Berlin, p 438
Heinze KG, Jahnz M, Schwille P (2004) Biophys J 86:506
Qian H, Elson EL (2004) Proc Nat Acad Sci USA 101:2828
Böhmer M, Wahl M, Rahn H-J, Erdmann R, Enderlein J (2002) Chem Phys Lett 353:439
Gösch M, Serov A, Anhut T, Lasser T, Rochas A, Besse PA, Popovic RS, Blom H, Rigler R (2004) J Biomed Opt 9:913
Gösch M, Blom H, Anderegg S, Korn K, Thyberg P, Wells M, Lasser T, Rigler R (2005) J Biomed Opt 10:054008
Takahashi Y, Sawada R, Ishibashi K, Mikuni S, Kinjo M (2005) Curr Pharm Biotechnol 6:159
Wong APY, Wiltzius P (1993) Rev Sci Instrum 64:2547
Cipelletti L, Weitz DA (1999) Rev Sci Instrum 70:3214
Viasnoff V, Lequeux F, Pine DJ (2002) Rev Sci Instrum 73:2336
Kannan B, Har JY, Liu P, Maruyama I, Ding JL, Wohland T (2006) Anal Chem 78:3444
Burkhardt M, Schwille P (2006) Opt Express 14:5013
Petersen NO, Hoddelius PL, Wiseman PW, Seger O, Magnusson KE (1993) Biophys J 65:1135
Brown CM, Petersen NO (1998) J Cell Sci 111:271
Vanden Broek W, Huang Z, Thompson NL (1999) J Fluoresc 9:313
Wiseman PW, Squier JA, Ellisman MH, Wilson KR (2000) J Microsc 200:14
Costantino S, Comeau JWD, Kolin DL, Wiseman PW (2005) Biophys J 89:1251
Hebert B, Costantino S, Wiseman PW (2005) Biophys J 88:3601
Kolin DL, Costantino S, Wiseman PW (2006) Biophys J 90:628
Kulkarni RP, Wu DD, Davis ME, Fraser SE (2005) Proc Nat Acad Sci USA 102:7523
Weissman M, Schindler H, Feher G (1976) Proc Nat Acad Sci USA 73:2776
Nicoli DF, Briggs J, Elings VB (1980) Proc Nat Acad Sci USA 77:4904
Petersen NO (1986) Biophys J 49:809
Koppel DE, Morgan F, Cowan AE, Carson JH (1994) Biophys J 66:502
Meyer T, Schindler H (1988) Biophys J 54:983
Berland KM, So PTC, Chen Y, Mantulin WW, Gratton E (1996) Biophys J 71:410
Ruan QQ, Cheng MA, Levi M, Gratton E, Mantulin WW (2004) Biophys J 87:1260
Skinner JP, Chen Y, Müller JD (2005) Biophys J 89:1288
Amediek A, Haustein E, Scherfeld D, Schwille P (2002) Single Mol 3:201
Digman MA, Brown CM, Sengupta P, Wiseman PW, Horwitz AR, Gratton E (2005) Biophys J 89:1317
Petrášek Z, Schwille P (2008) In: Rigler R, Vogel H (eds) Single molecules and nanotechnology. Springer, Berlin, p 83
Wang J, Sood AK, Satyam PV, Feng Y, Wu X-Z, Cai Z, Yun W, Sinha SK (1998) Phys Rev Lett 80:1110
Schrof W, Klingler JF, Rozouvan S, Horn D (1998) Phys Rev E 57:R2523
Hellerer T, Schiller A, Jung G, Zumbusch A (2002) ChemPhysChem 7:630
Brehm G, Sauer G, Fritz N, Schneider S, Zaitsev S (2005) J Mol Struct 735-736:85
Petrášek Z, Schwille P (2008) Biophys J 94:1437
Author information
Authors and Affiliations
Corresponding author
Editor information
Rights and permissions
Copyright information
© 2008 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Petrov, E.P., Schwille, P. (2008). State of the Art and Novel Trends in Fluorescence Correlation Spectroscopy. In: Resch-Genger, U. (eds) Standardization and Quality Assurance in Fluorescence Measurements II. Springer Series on Fluorescence, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2008_032
Download citation
DOI: https://doi.org/10.1007/4243_2008_032
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-70570-3
Online ISBN: 978-3-540-70571-0
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)