Abstract
The clinical use of human induced pluripotent stem cells (hiPSCs) and the development of patients-specific gene and cell therapies rely on the development of fast, reliable, and integration-free methods of derivation of pluripotent stem cells from somatic tissues. Here we describe an integration-free protocol for the rapid derivation of hiPSCs from dermal fibroblasts using modified mRNAs. This method is inexpensive, highly efficient, and makes use of reagents that are xeno-free and chemically defined and can therefore be adopted by any Good Manufacturing Practice (GMP) facility.
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Durruthy, J.D., Sebastiano, V. (2014). Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs. In: Turksen, K. (eds) Stem Cells and Good Manufacturing Practices. Methods in Molecular Biology, vol 1283. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_124
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DOI: https://doi.org/10.1007/7651_2014_124
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2434-9
Online ISBN: 978-1-4939-2435-6
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