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Isolation of Cancer Stem Cells from Squamous Cell Carcinoma

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Skin Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1879))

Abstract

Different cancer stem cell (CSC) populations can be found in many types of cancer, including squamous cell carcinoma (SSC). Diverse reports showed that CSC play a crucial role in the relapse of different types of cancer. CSC sustains tumor growth due to their capacity to self-renew and their potential to initiate secondary tumors with metastatic cancer features. Therefore, the development of methods for the isolation of CSC is a key step to explore the mechanisms underlying CSC maintenance. In this chapter, we provide a method for isolating CSC from cutaneous SSC using immunofluorescence labeling to allow the specific purification of CSC by fluorescence-activated cell sorting (FACS). This method is based on the use of CSC membrane markers, allowing as well the isolation CSC from different mouse strains.

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Acknowledgments

The Perez-Moreno lab is supported by grants from the Worldwide Cancer Research UK Foundation (15-1219) and the Novo Nordisk Foundation (NNF17OC0028028).

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Authors and Affiliations

  1. Section for Cell Biology and Physiology, Department of Biology, Faculty of Science, University of Copenhagen, Copenhagen Ø, Denmark

    Silvia Fontenete & Mirna Perez-Moreno

Authors
  1. Silvia Fontenete
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  2. Mirna Perez-Moreno
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Corresponding authors

Correspondence to Silvia Fontenete or Mirna Perez-Moreno .

Editor information

Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Fontenete, S., Perez-Moreno, M. (2018). Isolation of Cancer Stem Cells from Squamous Cell Carcinoma. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 1879. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_162

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  • DOI: https://doi.org/10.1007/7651_2018_162

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8869-3

  • Online ISBN: 978-1-4939-8870-9

  • eBook Packages: Springer Protocols

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