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Quantitative PCR Analysis of FosB mRNA Expression after Short Duration Oxygen and Light Stress

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Retinal Degenerations

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 533))

Abstract

Quantitative polymerase chain reaction (QPCR) was used to examine changes in FosB mRNA expression in models of oxygen and light stress to the retina. C57BL/6 mice or Sprague-Dawley (SD) albino rats were subjected to several experimental paradigms: short-term light or oxygen stress, extended hyperoxia (75% oxygen), or a model of oxygen-induced retinopathy (OIR). Control animals were subjected to room air and 5 lux cyclic light. FosB expression dramatically increases in response to light stress as well as in a model of OIR, but not in response to sustained 75% oxygen. These data suggest that both hypoxia and light stress induce expression of FosB in the retina.

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Author information

Authors and Affiliations

  1. School of Optometry, University of California, Berkeley, CA, 94720-2020, USA

    Scott F. Geller

  2. lnstitute for Biomedical Research and the Department of Anatomy and Histology, University of Sydney, Sydney, NSW, 2006, Australia

    Jonathan Stone

Authors
  1. Scott F. Geller
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  2. Jonathan Stone
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Editor information

Editors and Affiliations

  1. Beckman Vision Center, University of California, San Francisco, California, USA

    Matthew M. LaVail

  2. Cole Eye Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, USA

    Joe G. Hollyfield

  3. Dean A. McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    Robert E. Anderson

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© 2003 Springer Science+Business Media New York

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Geller, S.F., Stone, J. (2003). Quantitative PCR Analysis of FosB mRNA Expression after Short Duration Oxygen and Light Stress. In: LaVail, M.M., Hollyfield, J.G., Anderson, R.E. (eds) Retinal Degenerations. Advances in Experimental Medicine and Biology, vol 533. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0067-4_31

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  • DOI: https://doi.org/10.1007/978-1-4615-0067-4_31

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4909-9

  • Online ISBN: 978-1-4615-0067-4

  • eBook Packages: Springer Book Archive

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