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Cultured Rat Hepatocytes

  • Chapter
Book cover Models for Assessing Drug Absorption and Metabolism

Part of the book series: Pharmaceutical Biotechnology ((PBIO,volume 8))

Abstract

The use of in vitro and in vivo systems to evaluate hepatic drug uptake and metabolism, cytochrome P450 induction, drug interactions affecting hepatic metabolism, hepatotoxicity, and cholestasis is an essential part of the drug development process (Tavoloni and Boyer, 1980; Powis et al., 1989; Bertrams and Ziegler, 1991; Jurima-Romet and Huang, 1992; Komai et al., 1992; Jurima-Romet and Huang, 1993). Primary hepatocyte culture is one of many techniques used to address these issues. Although primary hepatocytes maintained under conventional culture conditions have been broadly used to study drug metabolism and hepatotoxicity, the rapid loss of liver-specific functions and the failure of cultured hepatocytes to reestablish normal bile canaliculi (cell polarity, cell architecture) have limited their application.

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Authors and Affiliations

  1. INTERx Research/Merck Research Laboratories, Lawrence, Kansas, 66047, USA

    Edward L. LeCluyse & Jerome H. Hochman

  2. Department of Pharmacology, Toxicology, and Therapeutics, University of Kansas Medical School, Kansas City, Kansas, 66160, USA

    Edward L. LeCluyse, Peter L. Bullock & Andrew Parkinson

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  1. Edward L. LeCluyse
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  3. Andrew Parkinson
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  1. The University of Kansas, Lawrence, Kansas, USA

    Ronald T. Borchardt

  2. SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania, USA

    Philip L. Smith

  3. Tacora Corporation, Seattle, Washington, USA

    Glynn Wilson

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LeCluyse, E.L., Bullock, P.L., Parkinson, A., Hochman, J.H. (1996). Cultured Rat Hepatocytes. In: Borchardt, R.T., Smith, P.L., Wilson, G. (eds) Models for Assessing Drug Absorption and Metabolism. Pharmaceutical Biotechnology, vol 8. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1863-5_9

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