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Pseudomonas Methods and Protocols pp 59–65Cite as

Plate-Based Assay for Swimming Motility in Pseudomonas aeruginosa

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1149))

Abstract

Swimming motility is a flagellum-dependent form of movement observed in the Gram-negative bacterium Pseudomonas aeruginosa. Swimming motility is defined as the movement in liquid or low-viscosity conditions (up to 0.3 % agar concentration). Unlike swarming motility, swimming motility requires a functional flagellum, but neither quorum sensing (QS) systems nor biosurfactants. While swimming motility can also be observed via microscopy, here we describe a reproducible plate-based method.

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References

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Acknowledgement

This work was supported by the NIH grant R01A1003256 to G.A.O. and the Rosaline Borison predoctoral fellowship awarded to D.G.H.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA

    Dae-Gon Ha, Sherry L. Kuchma & George A. O’Toole

Authors
  1. Dae-Gon Ha
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  2. Sherry L. Kuchma
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  3. George A. O’Toole
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Corresponding author

Correspondence to George A. O’Toole .

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Editors and Affiliations

  1. MRC Centre for Molecular Bacteriology and Infection (CMBI) Department of Life Sciences, Imperial College, London, UK

    Alain Filloux

  2. Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain

    Juan-Luis Ramos

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Ha, DG., Kuchma, S.L., O’Toole, G.A. (2014). Plate-Based Assay for Swimming Motility in Pseudomonas aeruginosa . In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_7

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  • DOI: https://doi.org/10.1007/978-1-4939-0473-0_7

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-0472-3

  • Online ISBN: 978-1-4939-0473-0

  • eBook Packages: Springer Protocols

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